通过替代拼接转录,CsTT8调节了血中安东的积累
Jianhui Wang1,2, Rui Xu1, Shuangping Qiu1
1Department of Food Science and Engineering, School of Food and Biological Engineering, Chengdu University, Chengdu 610106, China.
Horticulture research
|November 6, 2023
概括
血色基因CsTT8调节着色素的产生. 它的功能形式促进了氨酸合成,而一个拼接的变体,Δ15-TT8,可能会限制它,这表明水果颜色的关键比例.
科学领域:
- 植物分子生物学 植物分子生物学
- 果实生物化学 果实生物化学
- 基因规则 基因规则
背景情况:
- 安西是血的主要颜料,影响水果的颜色和营养价值.
- 基本螺旋环螺旋 (bHLH) 转录因子TT8在植物色素生物合成中起作用.
- 了解TT8在Citrus sinensis中的功能对于阐明安托素积累机制至关重要.
研究的目的:
- 为了识别和表征血的TT8同类 (Citrus sinensis cv"Tarocco").
- 调查CsTT8在色素生产中的替代拼接的作用.
- 阐明CsTT8调节氨酸生物合成和运输的分子机制.
主要方法:
- 在血色中鉴定和克隆CsTT8的基因.
- 定量实时PCR (qRT-PCR) 分析TT8和Δ15-TT8.8的mRNA表达水平.
- 亚细胞局部化试验和蛋白质-蛋白质相互作用研究 (例如,与RUBY1).
- 双露西法酶记者测定和烟草中的过度表达,以评估基因调节.
主要成果:
- 一个功能性的CsTT8基因和一个替代拼接变体 (Δ15-TT8) 在血色中被确定.
- 功能性TT8与Δ15-TT8mRNA水平的比率与水果组织中的色素存在相关.
- 功能性TT8定位在核中,与蛋白质相互作用,并通过调节复合体促进了素和酸盐的产生.
- Δ15-TT8缺乏核定位和相互作用能力,这表明它是一种非功能变体.
结论:
- 功能性TT8和替代拼接的Δ15-TT8之间的平衡对于血中的安托西亚宁积累至关重要.
- 功能性TT8,与MYB合作伙伴复合,积极调节氨酸生物合成和真空传输.
- 增加的 Δ15-TT8 可能起到负调节者的作用,通过反机制限制安托西亚尼水平.
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