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通过准miR-489-3p/S100A11轴来调节HUVEC细胞的LEF1-AS1机制

Haoran Zhang¹, Wenqiu Wang¹, Junjie Lin¹

¹Hemangioma Surgery Department, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, China.

|November 6, 2023

概括

长非编码RNA LEF1-AS1在静脉形中被上调,促进细胞增殖和迁移. LEF1-AS1针对miR-489-3p,增加S100A11的表达,并有助于静脉形的发展.

科学领域:

血管生物学血管生物学
分子遗传学分子遗传学
生物化学生化学

背景情况:

静脉形是常见的先天性血管异常,其特点是局部入侵和渐进的生长.
长非编码RNAs (lncRNAs) 在血管细胞功能和炎症中起着调节作用,影响静脉形的发展.

研究的目的:

为了研究 lncRNA LEF1-AS1 在静脉形致病的作用.
为了检查人静脉内皮细胞 (HUVEC) 中的LEF1-AS1,miR-489-3p和S100A11之间的相互作用.

主要方法:

使用 lncRNA 微阵列进行差异基因表达分析.
定量逆转录PCR (qRT-PCR) 用于RNA表达和西式涂抹用于蛋白质表达.
细胞检测包括CCK8,Transwell迁移和体外血管生成,以评估HUVEC功能.

主要成果:

与正常组织相比,LEF1-AS1在静脉形组织中显著上调.
生物信息分析预测了LEF1-AS1,miR-489-3p和S100A11之间的相互作用.
LEF1-AS1促进了HUVEC的增殖,迁移和血管生成,作为一个竞争的内源RNA (ceRNA) 来通过海绵式miR-489-3p.p.升调S100A11.

结论:

LEF1-AS1在静脉形上升调,并促进HUVEC的增殖,迁移和血管生成.
LEF1-AS1/miR-489-3p/S100A11轴与静脉形的发生有关.
这一监管轴为治疗静脉形提供了潜在的治疗点.

关键词:

在HUVECEC中使用.对于LEF1-AS1来说,这是一个很好的选择.在 S100A1111 中使用.静脉形静脉形发生在 miR-489-3p 中使用.

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