在纳米颗粒和平面表面支持的细胞膜涂层中保持侧面性和流动性
Sidi Liu1,2, Yuanfeng Li3,4, Linqi Shi4
1Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices, Institute of Functional Nano & Soft Materials (FUNSOM), Collaborative Innovation Center of Suzhou Nano Science and Technology, Soochow University, 199 Ren'ai Road, Suzhou, 215123, PR China.
Bioactive materials
|November 6, 2023
概括
支持的细胞膜涂层对纳米载体来说是有希望的. 水友糖残留物确保了正确的膜方向,而纳米结构设计提高了流动性,改善了生物活性材料的功能.
科学领域:
- 生物材料科学 生物材料科学
- 纳米技术纳米技术
- 表面化学 表面化学
背景情况:
- 支持的细胞膜对于生物活性纳米载体至关重要,但保持自然膜的特性,如侧向性和流动性是具有挑战性的.
- 了解支表面在保护膜完整性的作用,对于开发有效的纳米载体至关重要.
- 自然细胞膜表现出不对称的功能组分布 (侧面性) 和分子运动性 (流动性).
研究的目的:
- 审查自然膜特性和与支膜涂层相关的隔离方法.
- 为了确定维持膜侧面性和流动性的支表面特征.
- 为了阐明表面电荷和地形对支膜涂层特性的影响.
主要方法:
- 对自然膜性质的审查,包括侧面性和流动性.
- 对膜隔离技术的分析.
- 支持表面特性 (电荷,曲率,纳米结构) 和它们与细胞膜的相互作用的评估.
主要成果:
- 糖蛋白上的水友糖残留物正确地将细胞膜定向向支表面,确保侧向.
- 膜流动性在正确侧面涂层中保持在充电和未充电的表面上.
- 由于膜结,在小的,高度曲的纳米粒子和局部化的纳米结构上流动性降低.
- 纳米结构平面表面通过允许双面水性接触,促进了增强的流动性.
结论:
- 正确的膜侧面性是通过水友的外侧叶片相互作用实现的,独立于静电力.
- 膜流动性取决于表面地形和曲率,纳米结构平面表面具有优势.
- 优化支表面设计是利用纳米载体应用支细胞膜涂层的全部潜力的关键.
相关概念视频
01:23Membrane Fluidity
152.7K
Cell membranes are composed of phospholipids, proteins, and carbohydrates loosely attached to one another through chemical interactions. Molecules are generally able to move about in the plane of the membrane, giving the membrane its flexible nature called fluidity. Two other features of the membrane contribute to membrane fluidity: the chemical structure of the phospholipids and the presence of cholesterol in the membrane.
152.7K
01:35Asymmetric Lipid Bilayer
7.3K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.3K
01:18Membrane Domains
5.4K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.4K
01:34The Fluid Mosaic Model
148.4K
The fluid mosaic model was first proposed as a visual representation of research observations. The model comprises the composition and dynamics of membranes and serves as a foundation for future membrane-related studies. The model depicts the structure of the plasma membrane with a variety of components, which include phospholipids, proteins, and carbohydrates. These integral molecules are loosely bound, defining the cell’s border and providing fluidity for optimal function.
148.4K
01:19Fluid Mosaic Model
11.9K
Scientists identified the plasma membrane in the 1890s and its principal chemical components (lipids and proteins) by 1915. The model for plasma membrane structure, proposed in 1935 by Hugh Davson and James Danielli, was the first model to be widely accepted in the scientific community. The model was based on the plasma membrane's "railroad track" appearance in early electron micrographs. Davson and Danielli theorized that the plasma membrane's structure resembled a sandwich...
11.9K