相关实验视频

Updated: Jul 11, 2025

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses

Published on: December 29, 2015

日本脑炎病毒通过与不同的猪PML异型进行相互作用,扰乱PML核体

Songbai Yang¹, Huaijin Liu¹, Zhenyu Chen¹

¹Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China.

Frontiers in cellular and infection microbiology

|November 6, 2023

概括

日本脑炎病毒 (JEV) 感染减少了原细胞白血病蛋白核体 (PML-NBs). JEV非结构性蛋白与PML异型相互作用,破坏PML-NBs并可能削弱抗病毒免疫反应.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

亲细胞白血病 (PML) 蛋白及其核体 (PML-NBs) 对细胞功能和对病毒的天生的免疫力至关重要.
病毒通常针对PML-NBs来逃避宿主免疫检测.
日本脑炎病毒 (JEV) 导致严重的神经疾病,但其涉及PML-NBs的免疫逃避机制尚不清楚.

研究的目的:

研究JEV如何与PML-NBs相互作用以逃避宿主免疫反应.
为了确定涉及到PML-NBs中断的特定JEV蛋白质.
探索JEV蛋白与PML异型之间的相互作用及其对病毒复制的影响.

主要方法:

用JEV感染PK15细胞,并列出细胞内PML-NBs.
免疫光测试可视化PML-NBs和JEV抗原.
克隆并将十个JEV蛋白转化为PK15细胞.
JEV蛋白与猪PML异型一起感染.
在PML异型体的宫外表达时对JEV复制的评估.

主要成果:

疹病毒感染显著降低了受感染细胞中的PML-NBs数量.
发现5种JEV非结构性蛋白 (NS2B,NS3,NS4A,NS4B,NS5) 显著降低了PML-NB的数量.
特定的JEV蛋白与不同的PML异型 (例如,NS2B与PML4/PML5,NS3/NS5与所有异型) 呈现出明显的局部化模式.
PML异型PML1,PML3,PML4和PML5抑制了JEV的复制.

结论:

JEV通过与各种PML异形相互作用来破坏PML-NB结构.
这种干扰可能有助于JEV.逃避宿主的抗病毒免疫反应.
了解这些相互作用,可以深入了解JEV病原体和潜在的治疗点.

关键词:

日本脑炎病毒病毒.在PML的异构体中.在PML-NBs中,免疫光分析分析非结构性蛋白质的结构.

更多相关视频

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Using Reverse Genetics to Manipulate the NSs Gene of the Rift Valley Fever Virus MP-12 Strain to Improve Vaccine Safety and Efficacy

Published on: November 1, 2011

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Production of High-Titer Infectious Influenza Pseudotyped Particles with Envelope Glycoproteins from Highly Pathogenic H5N1 and Avian H7N9 Viruses

Published on: January 15, 2020

相关概念视频

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...