一个新的IncRNA介导的表观遗传调节机制在牙周炎
Zoe Xiaofang Zhu1,2, Yao Liu3, Jinghao Wang1
1Division of Oral Biology, Tufts University School of Dental Medicine, 136 Harrison Ave, M&V Building Room 830, Boston, MA 02111, United States.
International journal of biological sciences
|November 6, 2023
概括
长非编码RNAAPDC (lncR-APDC) 通过破坏免疫细胞功能和骨再生来加剧牙周炎. 恢复 lncR-APDC 显示了治疗牙周病的治疗潜力.
科学领域:
- 遗传学 遗传学 是一个
- 免疫学 免疫学 免疫学
- 口腔生物学 口腔生物学
背景情况:
- 牙周炎是一种普遍的炎症性疾病,导致组织破坏和牙损失.
- 像LncR-ANRIL这样的长非编码RNA (lncRNA) 与各种疾病有关,包括牙周炎.
- 作为人类LncR-ANRIL的老鼠 ортолог,LncR-APDC正在研究其在牙周炎中的作用.
研究的目的:
- 阐明 lncR-APDC 在实验性牙周炎 (EP) 进展中的调节功能.
- 确定与牙周炎中 lncR-APDC 缺乏相关的分子机制和细胞变化.
主要方法:
- 利用IncR-APDC基因淘汰赛 (KO) 的小鼠诱导实验性牙周炎.
- 在KO和野生型小鼠的牙组织上进行单细胞RNA测序.
- 分析了骨髓干细胞分化和免疫细胞种群.
- 研究了lncR-APDC和Tff2表达之间的相互作用.
主要成果:
- lncR-APDC-KO小鼠在EP中表现出恶化的骨损失和失调的炎症前驱细胞因子.
- 骨髓干细胞的骨质基因分化受损,来自IncR-APDC-KO小鼠.
- 在KO小鼠中,scRNA-seq揭示了免疫细胞组成和功能的改变,以及一种新的上皮细胞子集 (Krt8+/Krt18+).
- 在缺陷IncR-APDC的小鼠中,Tff2表达显著上调,有直接结合相互作用的证据.
结论:
- lncR-APDC是牙周炎进展的关键调节剂,影响骨代谢和免疫反应.
- 一个独特的上皮细胞子集和高的Tff2水平与牙周炎中的IncR-APDC缺乏有关.
- 使用AAV9-lncR-APDC的治疗干预在实验性牙周炎模型中显示出有效性,突出显示了其潜在的治疗价值.
相关概念视频
lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
Epigenetic Regulation
31.1K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
31.1K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Types of RNA
63.8K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
63.8K
Operons
49.1K
Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by...
49.1K


