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使用部分hsp65基因测序从假定结核病患者中识别显著的致病性非结核菌种

Dyshelly Nurkartika Pascapurnama¹, Nabilla Ghina Zavitri¹, Raspati Cundarani Koesoemadinata¹

¹Research Center for Care and Control of Infectious Diseases, Universitas Padjadjaran, Bandung, West Java, Indonesia.

Infection and drug resistance

|November 6, 2023

概括

热冲击蛋白65 (hsp65) 基因的部分基因测序提供了一种可靠的方法来识别非结核性真菌菌 (NTM) 物种,改善诊断超出临床和放射性评估.

科学领域:

微生物学微生物学
分子生物学分子生物学
传染性疾病传染性疾病

背景情况:

非结核菌 (NTM) 诊断传统上依赖于临床和放射学迹象.
准确的NTM物种识别对于有效治疗和管理NTM感染至关重要.

研究的目的:

评估部分热冲击蛋白65 (hsp65) 基因测序用于NTM物种识别的疗效.
为NTM建立基于序列的诊断方法.

主要方法:

传统的聚合酶连锁反应 (PCR) 从32个NTM分离物中放大hsp65基因.
针对性测序的PCR amplicons. 的目标测序.
使用DNA Baser对hsp65序列进行生物信息分析,并与GenBank NCBI数据库进行比较.

主要成果:

机会性NTM物种的鉴定,其中Mycobacterium abscessus (40.63%) 和Mycobacterium chelonae (18.75%) 是最常见的.
检测其他致病性NTM,包括M. fortuitum群,M. avium,M. 细胞内,M. kansasii和M. celatum.等.
证明hsp65基因在NTM特种化中的高分辨力.

结论:

部分hsp65基因测序是NTM物种化的一个有希望和可靠的替代方法.
这种基于序列的方法提高了NTM疾病的诊断能力.
这些发现特别适用于印尼标本中NTM识别.

关键词:

这就是HSP65基因.没有结核的真菌菌.基于序列的分析分析.标识物种标识物种标识物种

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