退行性宫肌病症在小鼠中诱导性别特异性失生症
Carlos Farkas1, Eduardo Retamal-Fredes2, Ariel Ávila2
1Biomedical Science Research Laboratory, Department of Basic Sciences, Faculty of Medicine, Universidad Católica de la Santísima Concepción, Concepción, Chile.
Frontiers in microbiology
|November 6, 2023
概括
退行性宫髓病变 (DCM) 改变了肠道微生物群,特别是在男性中,减少了有益的酸盐生产细菌. 这项研究揭示了与DCM病理生物学相关的性别特异性肠道微生物组变化.
科学领域:
- 神经科学是一个神经科学.
- 微生物学 微生物学
- 胃肠病学 胃肠病学
背景情况:
- 退行性宫髓病变 (DCM) 是老年人脊髓损伤的主要原因.
- 肠道微生物群通过微生物群-肠道-大脑轴影响中枢神经系统和免疫反应.
- 肠道微生物群在DCM病原发生中的作用在很大程度上仍未被探索.
研究的目的:
- 在小鼠模型中研究DCM对肠道微生物群组成和功能的影响.
- 探索DCM诱导的肠道失调的潜在性别特异性.
- 为了确定与DCM相关的特定微生物和代谢变化.
主要方法:
- 在C57BL/6小鼠中使用C5-6脊椎水平的外科压缩诱导DCM.
- 用16SrRNA测序分析便样本,以评估微生物群的组成.
- 流细胞计用于分析免疫细胞组成.
- 基因组规模代谢建模 (gapseq) 用于预测代谢途径.
主要成果:
- 与虚假对照相比,DCM在雄性和雌性小鼠中诱导了显著的肠道失调.
- 在雄性小鼠中,肠道失生症更为明显.
- 在DCM小鼠中,Lachnospiraceae和Muribaculaceae家族以及酸盐生产细菌的丰富程度发生了显著的变化.
- 代谢建模确定了改变的pyruvate-to-butanoate和pyruvate-to-propionate路径.
结论:
- DCM在肠道微生物群中诱导了动态的,性别特定的变化.
- 减少酸盐产生细菌的丰富性是DCM相关的肠道失生症的一个关键特征.
- 这些发现增强了对肠道微生物群在DCM病理生物学中的作用的理解.
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