兰默基因酶通过酸化MBF抑制剂Yox1来调节细胞循环,这种反应发生在Schizosaccharomyces pombe中
Kibum Park1, Joo-Yeon Lim2, Je-Hoon Kim1
1Laboratory of Cellular Differentiation, Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon, Republic of Korea.
Mycobiology
|November 6, 2023
概括
裂变酵母Lkh1激酶通过调节Mlu1细胞周期盒结合因子 (MBF) 转录复合体来调节细胞周期进展. Lkh1酸化Yox1,影响G1/S阶段过渡和细胞大小控制.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- Lkh1 是 * Schizosaccharomyces pombe * 中的一个 LAMMER 激酶,参与调节丝状生长,花和氧化应激反应.
- *lkh1*-删除突变体表现出表型,表明它在控制细胞大小和细胞周期进展方面发挥了作用.
研究的目的:
- 研究Lkh1在细胞循环调节中的作用.
- 阐明Lkh1影响细胞周期进展的分子机制.
主要方法:
- 对*lkh1*-删除突变的微阵列分析.
- 对Mlu1细胞周期盒结合因子 (MBF) 依存基因的转录分析.
- 拉下测试以确认蛋白质相互作用.
- 在体外激酶试验和NetPhosK 2.0分析.
主要成果:
- 微阵列分析显示,在lkh1*删除突变体中,MBF依赖细胞循环基因的上调调节.
- Lkh1与Yox1直接相互作用,Yox1是MBF的负调节器.
- 在氨酸残留物T40和T41上Lkh1酸化物Yox1
- 这种酸化调节MBF活动,影响G1/S阶段的进展.
结论:
- 在裂变酵母中,Lkh1在细胞循环调节中起着至关重要的作用.
- Lkh1通过对Yok1.1的直接相互作用和酸化来调节MBF活动.
- Lkh1-Yox1-MBF通路对于控制G1/S阶段过渡和细胞大小至关重要.
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