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抗菌支架的大规模开发:用于胃肠道应用的 Gentamicin 硫酸盐加载可生物降解的纳米纤维

Marketa Klicova¹, Senta Mullerova¹, Jachym Rosendorf²

¹Department of Nonwovens and Nanofibrous Materials, Faculty of Textile Engineering, Technical University of Liberec, Studentska 2, Liberec 461 17, Czech Republic.

|November 6, 2023

概括

这项研究开发了使用聚-ε-caprolactone 和 gentamicin 硫酸盐的大规模抗菌纳米纤维材料. 这些材料支持细胞生长,并有效抑制细菌感染,使它们适合医疗应用.

科学领域:

生物材料工程生物材料工程
纳米技术纳米技术
传染病研究传染病研究

背景情况:

现代医学需要先进的药物输送系统.
纳米纤维为新型治疗材料提供有前途的特性.
目前的实验室技术限制了可重现的表征,阻碍了研究.

研究的目的:

开发和测试大规模的抗菌纳米纤维材料.
为了预防细菌感染的术后并发症.
评估聚-ε-caprolactone与 gentamicin 硫酸盐用于伤口愈合的应用.

主要方法:

不用针的电聚-ε-caprolactone与不同度的甘胺硫酸盐.
元素分析,紫外线光谱测量和HPLC用于抗生素量化.
在体外细胞兼容性测试 (ISO 10993-5),细胞粘附/增殖试验 (CCK-8,光,SEM).
对 * Staphylococcus gallinarum * 和 * Escherichia coli * 的抗菌有效性测试.
对乙烯氧化物灭菌效应的评估.

主要成果:

定量检测证实了纳米纤维中存在胺硫酸盐.
纳米纤维材料表现出良好的细胞兼容性,并支持细胞粘附和增殖.
观察到有效抑制 * Staphylococcus gallinarum * 和 * Escherichia coli * 的生长.
乙烯氧化物灭菌保持了材料的细胞相容性和抗菌性质.

结论:

开发的纳米纤维材料是细胞兼容的,具有显著的抗菌活性.
对于这些先进的生物材料,通过无针电制造的大规模制造是可行的.
这些材料在医疗应用中显示出预防术后细菌感染的巨大潜力.

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