脂质酶脂酸:二糖醇酸转移酶的取决于基因基因的表达和功能影响藻的碳储存和生长
Yufang Pan1, Wanting Zhang1, Xiaofei Wang2
1Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China.
Plant physiology
|November 6, 2023
概括
藻 (Phaeodactylum tricornutum) 有两个脂基因:二糖醇转移酶 (PDAT) 基因基因基因基因基因,在三糖醇 (TAG) 合成和生长中发挥着不同的作用,特别是在压力下.
科学领域:
- 生物化学 生物化学
- 分子生物学分子生物学
- 藻类生物技术 藻类生物技术
背景情况:
- 植物,真菌和藻类中的三糖醇 (TAG) 合成涉及脂:二糖醇转酶 (PDAT).
- 藻 (Phaeodactylum tricornutum) 具有独特的PDAT基因,具有两种不同的等位基因,每个都有众多单核酸变异.
研究的目的:
- 调查P. tricornutum.中PDAT的等位基特异性表达和功能.
- 了解PDAT等位基因在TAG积累和在营养压力下生长中的作用.
主要方法:
- 对已发表的87个RNA测序数据集的分析和实验验证.
- 在P. tricornutum中过度表达PDAT等位基因,在酵母 (Saccharomyces cerevisiae) 中表达异质基因.
- 生物化学测定和PDAT的局部化研究.
主要成果:
- 一个PDAT基因 (基因基因基因二) 的表达是由低引发的,在压力下显著增强TAG积累 (44-74%).
- 与2等位基因相比,1等位基因的过度表达显示出最小的TAG增加和更大的生长抑制.
- PDAT基因组2呈现TAG生物合成活性,受特定的N端和C端氨基酸变异的影响,与基因组1不同.
结论:
- 在P. tricornutum中的PDAT基因基因在碳储存和生长调节方面具有不平等的功能,特别是在限制下.
- 这些不同的角色表明了改善生物技术应用的脂质和生物质生产的战略.
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