在面包小麦5B中剖析和验证一个有前途的QTL控制spikelet数
Yaoqi Si1, Shuiquan Tian1,2, Jianqing Niu1
1State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
概括
研究人员为小麦产量特征确定了五个稳定的定量特征位置 (QTL),包括每的尖端数量和头号的天数. 一个QTL,QSns.ucas-5B,得到了验证,并显示了通过分子育种提高小麦谷物产量的潜力.
科学领域:
- 植物遗传学和育种方法
- 农业科学 农业科学
- 定量特征位置 (QTL) 分析分析.
背景情况:
- 每个尖端的尖端数 (SNS) 和天到头 (DTH) 对于提高小麦产量至关重要.
- 开发高密度遗传地图对于识别控制小麦重要的农学特征的基因至关重要.
研究的目的:
- 使用单核酸多态 (SNP) 标记器构建一个高密度的遗传图.
- 确定和验证小麦中SNS和DTH的环境稳定的定量特征位点 (QTL).
主要方法:
- 构建一个高密度的遗传地图,利用来自Wheat660K SNP阵列中的95,444个SNP标记,在一个重组杂交系 (RILs) 群体中.
- 确定SNS和DTH的五个环境稳定的QTL.
- 验证一个特定的QTL,QSns.ucas-5B,在多个环境中使用残留异合体线.
主要成果:
- 总共有五个与SNS和DTH相关的QTL被确定.
- 位于5BL染色体臂上的QTL QSns.ucas-5B得到了验证.
- 在QSns.ucas-5B内部的一个精英单元型积极影响了SNS和谷物产量组件,而没有延迟出发时间.
结论:
- 鉴定的QTLs,特别是QSns.ucas-5B,为未来的基因克隆工作提供了基础,以提高小麦产量.
- 经过验证的QSns.ucas-5B的InDel标记物具有在旨在增加小麦谷物产量的分子育种计划中应用的重大潜力.
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