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Updated: Jul 11, 2025

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
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使用pho-lac报告器融合定义膜蛋白拓学

Gouzel Karimova1, Daniel Ladant2

  • 1Unité de Biochimie des Interactions Macromoléculaires, Département de Biologie Structurale et Chimie, Institut Pasteur, CNRS, UMR 3528, Université Paris Cité, Paris, France. gouzel.karimova@pasteur.fr.

Methods in molecular biology (Clifton, N.J.)
|November 6, 2023
PubMed
概括

这项研究引入了Pho-Lac双记者系统,用于在体内确定膜蛋白拓. 该系统使用性酸酶和β-酸酶活动可靠地绘制细菌细胞内的蛋白质位置.

关键词:
双记者系统是双记者系统.膜蛋白是一种膜蛋白.膜拓学 膜拓学 膜拓学酸酶是一种酸酶.这就是β-galactosidase.

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