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Updated: Jul 11, 2025

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
11:33

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking

Published on: December 17, 2013

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在活体中,对外包蛋白的位置定向和时间分辨率的光交叉链接

Yassin A Abuta'a1, Anne Caumont-Sarcos1, Cécile Albenne1

  • 1Laboratoire de Microbiologie et Génétique Moléculaires (LMGM), Centre de Biologie Intégrative (CBI), Université de Toulouse, CNRS, UPS, Toulouse, France.

Methods in molecular biology (Clifton, N.J.)
|November 6, 2023
PubMed
概括

站点导向的光交联显示了大肠杆菌包膜蛋白相互作用. 这种方法识别了原生细胞环境中的暂时和稳定的蛋白质-蛋白质相互作用.

关键词:
细菌包裹是一种细菌包裹.埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.质谱测量质量谱测量蛋白质分泌 蛋白质分泌蛋白质脂质相互作用蛋白质蛋白质相互作用网站指导的照片交叉链接.

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Examining the Conformational Dynamics of Membrane Proteins in situ with Site-directed Fluorescence Labeling

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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