Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Structural basis for synthase activation and cellulose modification in the E. coli Type II Bcs secretion system.

Nature communications·2024
Same author

Bacterial synthase-dependent exopolysaccharide secretion: a focus on cellulose.

Current opinion in microbiology·2024
Same author

Structures and roles of BcsD and partner scaffold proteins in proteobacterial cellulose secretion.

Current biology : CB·2023
Same author

Bacterial crystalline cellulose secretion via a supramolecular BcsHD scaffold.

Science advances·2022
Same author

Weaving of bacterial cellulose by the Bcs secretion systems.

FEMS microbiology reviews·2021
Same author

MagC is a NplC/P60-like member of the α-2-macroglobulin Mag complex of Pseudomonas aeruginosa that interacts with peptidoglycan.

FEBS letters·2021

相关实验视频

Updated: Jul 4, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

22.3K

大型综合体:克隆策略,生产和净化

Samira Zouhir1,2,3, Wiem Abidi1,2,4, Petya V Krasteva5,6

  • 1Université de Bordeaux, CNRS, Bordeaux INP, CBMN, UMR 5248, Pessac, France.

Methods in molecular biology (Clifton, N.J.)
|November 6, 2023
PubMed
概括

本研究详细介绍了克隆,表达和净化细菌纤维素分泌 (Bcs) 大分子组合的方法. 这些策略使复杂的细菌纳米机器的结构功能研究能够用于生物技术应用.

关键词:
不同类的表达式 不同类的表达式宏分子复合体是什么?蛋白质复合体是一种蛋白质复合体.蛋白质生产 蛋白质生产蛋白质净化 蛋白质的净化

更多相关视频

The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.0K
Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

17.5K

相关实验视频

Last Updated: Jul 4, 2026

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics
07:48

A High-throughput Automated Platform for the Development of Manufacturing Cell Lines for Protein Therapeutics

Published on: September 22, 2011

22.3K
The MultiBac Protein Complex Production Platform at the EMBL
13:51

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

16.0K
Laboratory Scale Production and Purification of a Therapeutic Antibody
09:54

Laboratory Scale Production and Purification of a Therapeutic Antibody

Published on: January 24, 2017

17.5K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 微生物学 微生物学

背景情况:

  • 细菌,通常是单细胞 prokaryotes,使用宏分子复合体区分细胞过程.
  • 这些复合体控制各种功能,包括细胞分裂,运动和代谢道.
  • 同调节的基因集群促进了这些功能复合体的组装.

研究的目的:

  • 介绍细菌纤维素分泌 (Bcs) 大分子组件的克隆,表达和净化策略.
  • 为了使这些复杂的纳米机器的结构功能研究.
  • 为各种多组分细菌组件提供可适应的方法.

主要方法:

  • 克隆特定路径的基因集群.
  • 多元组件系统的重组联合表达.
  • 完整的宏分子组件的净化,用于结构分析.

主要成果:

  • 成功克隆,表达和净化Bcs组合.
  • 适用于各种细胞和膜嵌入组件的策略的演示.
  • 促进医学和生物技术相关性的结构功能研究.

结论:

  • 提出的策略对于研究复杂的细菌宏分子组合是有效的.
  • 这些方法可以适应涉及细菌生理学的各种纳米机器.
  • 这项工作支持了对细菌系统的理解和工程学的进展.