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相关概念视频

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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相关实验视频

Updated: Jul 11, 2025

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

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通过冷电子显微镜对蛋白质复合物的结构分析.

Athanasios Ignatiou1, Kévin Macé1, Adam Redzej1

  • 1Institute for Structural and Molecular Biology, School of Biological Sciences, Birkbeck College, London, UK.

Methods in molecular biology (Clifton, N.J.)
|November 6, 2023
PubMed
概括

单颗粒冷电子显微镜 (cryo-EM) 现在可以为各种生物复杂物实现近原子分辨率. 本综述详细介绍了用于样本准备,数据收集和图像分析的冷EM方法,强调了研究细菌分泌系统等灵活系统的应用.

科学领域:

  • 结构生物学是结构生物学.
关键词:
低温电子显微镜的使用方法图像处理 图像处理样品的准备 样品的准备单个粒子分析第四类分泌系统 IV型分泌系统

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Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Single Particle Cryo-Electron Microscopy: From Sample to Structure

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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

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相关实验视频

Last Updated: Jul 11, 2025

High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
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High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis

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Single Particle Cryo-Electron Microscopy: From Sample to Structure
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Single Particle Cryo-Electron Microscopy: From Sample to Structure

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Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
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  • 生物物理学的生物物理.
  • 分子成像学分子成像学

    • 背景情况:

    • 单粒子冷电子显微镜 (cryo-EM) 已经成为一种强大的技术,与X射线晶体学竞争,用于生物复杂物的结构确定.
    • 数字成像和图像处理算法的进步使近原子分辨率 (1.43 Å) 对广泛的生物宏分子成为可能.
    • 虽然像阿波费里丁这样的稳定复合体具有良好的分辨率,但灵活和多组件系统,如细菌分泌系统,存在重大结构特征挑战.

    结论:

    • 低温电磁波是一种多功能且越来越不可或缺的工具,用于阐明复杂生物系统的结构.
    • 描述的工作流为研究人员提供了一个框架,以解决具有挑战性的结构生物学问题,包括分析动态蛋白质机械.
    • 这些方法的进一步应用将继续推动我们对生物学中的分子机制的理解.