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Updated: Jul 11, 2025

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

Published on: January 3, 2012

效应器转位试验:差分溶解化试验

Irina S Franco^1,2, Sara V Pais², Nuno Charro²

¹Associate Laboratory i4HB - Institute for Health and Bioeconomy, NOVA School of Science and Technology, NOVA University Lisbon, Caparica, Portugal.

Methods in molecular biology (Clifton, N.J.)

|November 6, 2023

概括

此摘要是机器生成的。

在宿主细胞中识别细菌效应蛋白是了解病原体毒性的关键. 一种新的方法使用Triton X-100洗剂将这些蛋白质从感染的哺乳动物细胞中分离出来进行分析.

关键词:

细菌蛋白质分泌系统是一种细菌蛋白质分泌系统.洗剂的溶解化溶解.效应器;转移位置免疫阻塞是指免疫阻塞.这是一个SDS-PAGE页面.第三类分泌的分泌物.

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Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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Last Updated: Jul 11, 2025

Detection of Toxin Translocation into the Host Cytosol by Surface Plasmon Resonance

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Published on: January 3, 2012

Applying Fluorescence Resonance Energy Transfer FRET to Examine Effector Translocation Efficiency by Coxiella burnetii during siRNA Silencing

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科学领域:

微生物学微生物学
细胞生物学细胞生物学
病原体与宿主之间的相互作用

背景情况:

细菌病原体利用复杂的纳米机器将效应蛋白注入宿主细胞.
了解这些注射的蛋白质对于阐明细菌毒性机制至关重要.

研究的目的:

描述一种用于从感染的哺乳动物宿主细胞中分离细菌效应蛋白的新方法.
为了促进蛋白质转位和宿主细胞被细菌病原体操纵的研究.

主要方法:

使用哺乳动物组织培养感染模型.
使用Triton X-100洗剂来选择性地溶解宿主细胞膜.
隔离一种无细菌的,在宿主细胞蛋白中丰富的Triton可溶性分离物.

主要成果:

该方法成功地将细菌成分与宿主细胞成分分开.
隔离的部分富含来自宿主细胞细胞质,细胞核和血的蛋白质.
免疫洗可以用于检测该分数内的特定细菌效应蛋白.

结论:

这种技术为识别转移到宿主细胞中的细菌效应蛋白提供了一个强大的方法.
它有助于剖析细菌病原和毒性的分子机制.
该方法对于研究病原体与宿主相互作用和开发有针对性的干预措施是有价值的.