通过扫描电子显微镜进行细胞内结构的三维分析
Daisuke Koga1, Satoshi Kusumi2, Hirokazu Yagi3,4
1Department of Microscopic Anatomy and Cell Biology, Asahikawa Medical University, 2-1-1-1 Midorigaoka-higashi, Asahikawa 078-8510, Japan.
Microscopy (Oxford, England)
|November 6, 2023
概括
奥斯粉碎允许使用扫描电子显微镜 (SEM) 直接对细胞膜进行3D超结构观测. 最近的进展为增强生物和生物医学研究提供了快速,结合和相关的技术.
科学领域:
- 细胞生物学 细胞生物学
- 显微镜的使用方法
- 生物技术是生物技术.
背景情况:
- 超薄部分的二维成像提供了有限的洞察力膜性有机体的3D形态.
- 对膜系统的直接3D超结构观测对于理解细胞结构至关重要.
研究的目的:
- 审查和讨论用于扫描电子显微镜 (SEM) 的奥斯磨方法的新进展.
- 探索这些改进技术在生物学和生物医学研究中的潜力.
主要方法:
- 奥斯消磨:一种技术,通过去除可溶性细胞质蛋白质来保存膜质器官.
- 开发了快速的氧化,阿加罗斯嵌入组合和相关的免疫光-SEM技术.
主要成果:
- 氧化法使得高尔基器官,线粒体和内质网膜等器官的直接3D可视化成为可能.
- 新的技术提高了奥斯米消磨的速度,适用于不同类型的细胞,以及相对应的功能.
结论:
- 先进的氧磨技术显著扩大了研究3D细胞超结构的可能性.
- 这些方法对未来的生物和生物医学研究有很大的潜力.
更多相关视频
08:46Author Spotlight: A Three-Dimensional Technique for the Visualization of Mitochondrial Ultrastructural Changes in Pancreatic Cancer Cells
Published on: June 23, 2023
1.7K
06:453D Mitochondrial Ultrastructure of Drosophila Indirect Flight Muscle Revealed by Serial-section Electron Tomography
Published on: December 19, 2017
8.6K
相关概念视频
01:28Three-Dimensional Microscopy in Microbiology
47
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
47
01:07Electron Microscope Tomography and Single-particle Reconstruction
2.4K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.4K
01:17Studying the Cytoskeleton
6.3K
The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
6.3K