PICKLE RELATED 2是一种新功能化的基因复制物,在积极选择下,对种子转录组上的祖先PICKLE基因产生敌对作用
Gilles Dupouy1, Ronan Cashell1, Galina Brychkova1
1Genetics and Biotechnology Lab, Agriculture & Bioeconomy Research Centre, Ryan Institute, University of Galway, Galway H91 REW4, Ireland.
Genome biology and evolution
|November 6, 2023
概括
基因重复导致了Brassicaceae中的PIKLE (PKL) 和PIKLE相关的2 (PKR2) 蛋白. 这些CHD3蛋白质以不同的方式进化,对阿拉比多普西斯种子发育产生敌对影响.
科学领域:
- 植物分子生物学 植物分子生物学
- 进化遗传学的进化遗传学
- 转录法规 转录法规
背景情况:
- 染色体重塑蛋白质,特别是CHD3家族,对于真核细胞的细胞命运决定至关重要.
- CHD3蛋白质PIKKLE (PKL) 和PIKKLE-RELATED 2 (PKR2) 在阿拉比多普西斯莉亚种子发育中表现出对抗性的作用.
- 对于PKL和PKR2的功能分歧的进化基础仍然不清楚.
研究的目的:
- 研究PKL和PKR2基因的进化起源和发展轨迹.
- 确定它们在种子发育中的对抗性功能背后的分子机制.
- 了解基因重复和新功能化如何塑造蛋白质的进化和功能.
主要方法:
- 对CHD3家族基因的遗传学分析.
- 在PKL和PKR2同类体上检测阳性和净化选择.
- 蛋白质内在障碍的分析.
- 对pkr2和pkl突变体的全基因组转录组分析.
主要成果:
- PKL和PKR2起源于皇冠Brassicaceae中的基因复制事件.
- 在积极选择下,PKR2经历了快速进化,而PKL则经历了净化选择.
- 对PKR2的积极选择与减少的内在蛋白质障碍相关.
- 转录组分析揭示了种子中PKL和PKR2对特定基因的对抗性调节.
结论:
- 基因重复和随后的新功能化推动了CHD3家族中对抗性功能的进化.
- 对PKL和PKR2的明显进化压力有助于它们在植物繁殖中的特殊作用.
- 了解这些进化动态,可以了解蛋白质多样化及其对发育的影响.
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