通过用塑料降解微生物强化肠道微生物群,提高地 (Lumbricus terrestris) 对塑料污染的耐受性
Victor Carpena-Istan1, Macarena M Jurado1, Maria J Estrella-Gonzalez1
1Department of. Biology and Geology, CITE II-B, University of Almeria, Agrifood Campus of International Excellence, ceiA3, CIAIMBITAL, ctra. Sacramento s/n, 04120, Almeria, Spain.
Journal of hazardous materials
|November 6, 2023
概括
土虫益生菌,Pseudomonas alkylphenolica和Pseudomonas putida,增强了塑料污染土壤中的生存能力. 这些微生物通过促进有益的肠道细菌,帮助塑料生物修复.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 土虫生态学 土虫生态学
背景情况:
- 塑料污染对陆地生态系统构成重大威胁.
- 虫在土壤健康和营养循环中发挥着至关重要的作用.
- 虫的肠道微生物群可以受到塑料等环境污染物的影响.
研究的目的:
- 研究L. terrestris与肠道相关的微生物在塑料污染的土壤中作为地的益生菌的潜力.
- 识别和选择具有塑料降解酶能力的特定微生物菌株.
- 为了评估这些益生菌对子生存,体重和肠道微生物组合在中宇宙试验中的影响.
主要方法:
- 从曾经接触过各种塑料 (PET,LDPE,LLDPE,PS) 的虫肠道中分离和识别微生物.
- 选择 Pseudomonas alkylphenolica (PL4) 和 Pseudomonas putida (PL5) 菌株作为接种剂.
- 使用塑料污染土壤 (LDPE,LLDPE,回收皮) 与用益生菌治疗的地进行中宇宙规模的试验.
- 基于培养的 (cetrimide agar) 和分子 (qPCR) 方法用于追踪注射剂的存在.
- 进行metataxonomic分析,以评估地微生物群的生物多样性和功能.
主要成果:
- 益生菌的应用显著改善了塑料污染土壤中的地生存率.
- 选择的益生菌菌株P.alkylphenolica (PL4) 和P.putida (PL5) 在虫肠道中成功追踪.
- 经过metataxonomic分析,发现益生菌处理的虫中,与塑料生物修复相关的微生物群体的丰富性增加.
- 土虫的体重和整体微生物群的功能受到益生菌治疗的积极影响.
结论:
- 从肠中分离出来的微生物可以作为有效的益生菌,在塑料污染的环境中增强的弹性.
- 使用P.alkylphenolica和P.putida作为接种剂可以加强虫的肠道微生物群,有助于塑料生物修复.
- 这种方法为减轻土壤中塑料污染对生态的影响提供了一个有希望的战略.
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