现型变异诱导了Bacillus subtilis生物膜生长中的方向顺序和形态的出现
Jin Li1, Jin Wu1, Jiankun Wang1
1School of Mechanical Engineering, University of Science and Technology Beijing, Beijing, 100083, China.
Biochemical and biophysical research communications
|November 6, 2023
概括
细菌细菌生物膜形成有序的结构,以增强生存. 研究人员发现,控制环境摩擦和细胞粘附可以调节生物膜生长.
科学领域:
- 微生物学 微生物学
- 生物物理学的生物物理.
- 系统生物学 系统生物学
背景情况:
- 细菌细菌生物膜表现出复杂,有序的结构,对生存和适应性至关重要.
- 细菌的方向影响生物膜形态和集体行为,区分生物膜和浮游生物状态.
研究的目的:
- 调查不同Bacillus subtilis表型对生物膜结构和集体行为的影响.
- 探索细菌定向,生物膜形态和环境适应性之间的关系.
- 通过操纵环境和细胞因素来开发一种控制生物膜生长的模型.
主要方法:
- 在MSgg基底上观察Bacillus subtilis生物膜的生长.
- 分析不同的细菌表型 (流动性与矩阵生成细胞) 以及它们对生物膜架构的贡献.
- 开发和应用一个结合粗粒度和基于个体的模型.
主要成果:
- 运动细胞形成同位素,圆形的生物膜,而矩阵生成细胞形成链条,引导运动细胞进入阴性阶段.
- 由两种细胞类型组成的生物膜表现出不规则的形态.
- 模型模拟表明,环境摩擦,生长速度和细胞粘附是生物膜发育的关键调节因素.
结论:
- 细菌的定向顺序对于形成结构化的Bacillus subtilis生物膜具有增强的移动性和适应性至关重要.
- 特定的细胞表型决定了生物膜形态和集体行为.
- 可以调节环境和细胞参数,以控制生物膜生长动态.
相关概念视频
Microbial Morphologies
23
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
23
Biofilms
29
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
29
Bacterial Phylum Actinobacteria
19
Coryneform bacteria are gram-positive, aerobic, nonmotile rods that exhibit irregular, club-shaped, or V-shaped arrangements. Their V-shape results from snapping division, where the inner cell wall layer forms the cross-wall, while the outer layer remains intact until it ruptures on one side, causing the daughter cells to bend away.The primary genera are Corynebacterium and Arthrobacter. Corynebacterium includes diverse species, ranging from saprophytes to pathogens like Corynebacterium...
19
Fimbriae, Pili, and Axial Filaments
29
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
29
Bacterial Phylum Planctomycetes
15
Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
15
Bacterial Phylum Bacteroidota
24
The phylum Bacteroidota includes over 700 species classified into four primary orders: Bacteroidales, Cytophagales, Flavobacteriales, and Sphingobacteriales. These gram-negative, non-sporulating rods exhibit saccharolytic capabilities and can be aerobic or fermentative, encompassing obligate aerobes, facultative aerobes, and obligate anaerobes. Many species display gliding motility, though some are nonmotile or use flagella. The genus Bacteroides is well-studied due to its significant role in...
24


