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使用定制的酶尾酒加强玉米纤维糖化

Julen Ordeñana Manso¹, Martin B Nielsen¹, Eva Balaguer Moya¹

¹Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Building 223, 2800, Kongens Lyngby, Denmark.

Enzyme and microbial technology

|November 6, 2023

概括

开发一个定制的酶尾酒有效地将玉米纤维分解为用于生物制造的糖. 这个过程优化了糖化效率,这对于可持续的循环经济和释放生物质潜力至关重要.

科学领域:

生物技术是生物技术.
生物质的价值化生物质的价值化
酶学是一种酶学.

背景情况:

转向循环经济需要创新的方法来利用纤维素生物质.
富含粉的玉米纤维与传统生物质相比,为生物制造提供了独特的碳水化合物潜力.
高效地将玉米纤维转化为可发酵糖是其可持续应用的关键.

研究的目的:

开发一个量身定制的酶尾酒,以有效地将玉米纤维糖化为单糖.
研究酶的最佳组合和剂量,以最大限度地释放糖分.
为了应对玉米纤维的复原性和独特成分所带来的挑战.

主要方法:

玉米纤维经过温和的热水预处理 (在100°C下30分钟).
一种由Cellic® CTec2,Pentopan® Mono BG和Termamyl® 300 L组成的尾酒被用于水解.
实验设计被用来优化酶组合和剂量用于糖化.

主要成果:

最佳的酶组合 (60 FPU/g DM Cellic® CTec2和4.62 U/g DM Termamyl® 300 L,没有Pentopan® Mono BG) 产生了最高的葡萄糖 (66%) 和树脂糖 (30%) 释放.
较高的酶剂量显示效率回报减少,同时增加成本.
玉米纤维固有的回收性限制了完全的酶降解.

结论:

一种特定的酶尾酒,结合预处理,对于高效的玉米纤维糖化至关重要.
建议进一步优化预处理步骤,以进一步降低回收性并提高酶尾酒的性能.
这种量身定制的方法促进了玉米纤维在基于发酵的生物制造工艺中的利用.

关键词:

细胞质酶是一种细胞质酶.玉米纤维是一种玉米纤维.设计实验的设计.酶性糖化是一种酶性糖化.半细胞酶是一种半细胞酶.

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