来自Eimeria necatrix的CAP域的SAG蛋白的表达及其在入侵和免疫保护中的作用
Feiyan Wang1, Amin Zhang1, Xuelian Fan1
1College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety, the Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Veterinary parasitology
|November 6, 2023
概括
这项研究确定了Eimeria necatrix表面抗原CAP (EnSAG-CAP) 作为潜在的菌病疫苗候选人. 重组EnSAG-CAP在中引起了保护性免疫力,证明了其对抗Eimeria necatrix的疫苗开发潜力.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 埃梅里亚尼卡特里克斯是一种重要的家禽病原体,造成了相当大的经济损失.
- 包括Eimeria在内的Apicomplexa的表面抗原 (SAG) 对寄生虫与宿主相互作用至关重要.
- 对于Eimeria necatrix的糖酸氨基醇 (GPI) 链接的表面蛋白质,人们知之甚少.
研究的目的:
- 为了描述Eimeria necatrix sag基因 (Ensag-CAP) 和其编码的蛋白质.
- 研究EnSAG-CAP在E. necatrix中的局部化和功能.
- 评估复合EnSAG-CAP (rEnSAG-CAP) 对E.necatrix感染的免疫保护潜力.
主要方法:
- 基因克隆和Ensag-CAP的重组蛋白质表达.
- 西方斑点和间接免疫光测定用于蛋白质检测和定位.
- 用PI-PLC进行酶消化以确认GPI.
- 实时定量PCR (qPCR) 分析基因表达.
- 试管体内杂虫入侵测定和体内免疫研究在.
主要成果:
- 恩萨格-CAP基因编码了一种270氨基酸蛋白质,具有CAP域.
- 重组ENSAG-CAP (rEnSAG-CAP) 已成功表达并被特定抗体识别.
- 证实EnSAG-CAP是一种GPI定蛋白,位于E. necatrix merozoites的表面.
- 在第二代 merozoites (MZ-2) 中,EnSAG-CAP 表达显著高于在 schizonts (SZ) 中.
- rEnSAG-CAP在中对E. necatrix挑战产生了显著的免疫保护,由高ACI值和升高的IgY反应表明.
结论:
- EnSAG-CAP是一种E. necatrix的表面局部化,GPI固的蛋白质.
- EnSAG-CAP在杂虫入侵中发挥着重要作用.
- 再组合的EnSAG-CAP作为一种候选抗原,有望用于开发针对菌病的亚单元疫苗.
相关概念视频
Immunoglobulin-like Cell Adhesion Molecules
3.3K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
3.3K
Overview of Secretory Vesicles
8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K


