工程Yarrowia lipolytica作为一个平台菌株,用于从可再生资源中生产酸
Sang Min Lee1, Ju Young Lee2, Ji-Sook Hahn3
1Center for Bio-based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Ulsan 44429, Republic of Korea; Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 08826, Republic of Korea.
Bioresource technology
|November 6, 2023
概括
这项研究设计了Yarrowia lipolytica以产生生物基的酸,这是一个关键的二碳酸. 经过修改的酵母实现了9.7倍的增长,从可再生资源的酸生产.
科学领域:
- 生物技术是生物技术.
- 代谢工程是代谢工程.
- 工业微生物学 工业微生物学
背景情况:
- 作为石油化学品的替代品,对可持续的生物基二碳酸 (DCAs) 的需求日益增长.
- 亚酸是工业上必不可少的DCA,不是自然由微生物合成的.
- 雅罗维亚脂质菌提供了一个有前途的平台,用于微生物生产有价值的化学物质.
研究的目的:
- 设计一个Yarrowia lipolytica平台菌株,以有效地将可再生原料转化为酸.
- 通过操纵参与其合成和降解的代谢途径来增强酸生产.
- 建立一个强大的生物工艺,从脂肪酸甲基中生产酸.
主要方法:
- 对Yarrowia lipolytica进行基因工程,通过选择性淘汰乙-CoA氧化酶来破坏过氧体β氧化.
- 通过过度表达cytochrome P450单氧化酶 (ALK5),减少酶 (CPR1) 和脂肪醇氧化酶 (FAO1) 来增强 ω氧化途径.
- 优化使用SP92D介质生产酸的两阶段生物转化过程.
主要成果:
- 成功设计了Yarrowia lipolytica,以防止亚酸的完全氧化.
- 与父母菌株相比,酸产量显著增加了9.7倍.
- 通过使用工程Y. lipolytica.证明了第一次从脂肪酸甲基中成功生产酸.
结论:
- 工程Yarrowia lipolytica是一种可行的平台,用于可持续生产脂肪酸.
- 针对β-氧化和ω-氧化的代谢工程策略对于提高酸产量是有效的.
- 这项工作代表了从可再生资源中生物基酸的微生物合成的重大进展.
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