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miR-124-3p调节了Ptpn1在小鼠丸发育和精子生成中的参与

Lvjing Luo¹, Lishuang Sun², Shu Li¹

¹Youjiang Medical University for Nationalities, Baise, Guangxi 533000, China.

|November 6, 2023

概括

蛋白质铁酸酶,非受体类型1 (Ptpn1) 在成年小鼠丸中被上调. 微RNA-124-3p (miR-124-3p) 负面调节Ptpn1,这表明它在男性生殖细胞发育和Ptpn1表达控制中的作用.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
基因规则基因规则

背景情况:

丸发育和精子生成涉及复杂的基因调节,包括非编码RNA和酸化.
蛋白氨酸酸酶,非受体类型1 (Ptpn1) 是一种广泛表达的酶,在这些过程中具有潜在的作用.

研究的目的:

研究Ptpn1的表达及其与小鼠丸发育中的miR-124-3p的调节关系.
阐明miR-124-3p在调节男性生殖细胞中Ptpn1表达中的作用.

主要方法:

生物信息学分析
实时定量PCR (RT-qPCR) 是一种实时定量PCR.
西部污染 (WB) 是指西方污染.
免疫光 (IF) 是一种
在小鼠spermatogonia中进行细胞传染研究.

主要成果:

与青少年丸相比,成年老鼠丸中的Ptpn1mRNA和蛋白质水平更高.
miR-124-3p的表达与Ptpn1的表达呈现出反向相关性.
Ptpn1蛋白在男性生殖细胞的细胞质中局部化,在精子中表达更高.
在小鼠精子中,miR-124-3p的过度表达显著降低了Ptpn1mRNA和蛋白质水平.
生物信息学表明,Ptpn1通过脱化参与蛋白激酶失活.

结论:

随着小鼠丸的成熟,Ptpn1的表达增加.
miR-124-3p在小鼠精子中作为Ptpn1的负调节剂.
在精子生成过程中,miR-124-3p/Ptpn1轴可能对调节基因表达至关重要.

关键词:

缺乏酸化的方法在Ptpn1上使用.精子发生是精子生成.丸的发展丸的发展在 miR-124-3p 中使用.

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