转录组分析揭示了NETs激活对气道上皮质细胞EMT和支气管炎 obliterans炎症炎症的影响
Zhongji Wu1, Xiaowen Chen1, Shangzhi Wu1
1The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510000, People's Republic of China.
Scientific reports
|November 6, 2023
概括
中性细胞细胞外陷 (NETs) 通过激活炎症和上皮细胞转移到介质细胞的过渡途径,促进支气管炎消灭症 (BO). 这项研究确定了NETs作为BO病变发生的关键因素,提供了潜在的治疗点.
科学领域:
- 肺部医学 肺部医学
- 免疫学 免疫学 免疫学
- 分子生物学分子生物学
背景情况:
- 支气管炎 obliterans (BO) 是一种具有未知的分子机制的慢性呼吸道疾病.
- 中性细胞外细胞陷 (NETs) 与炎症性疾病有关,但它们在BO中的作用尚不清楚.
研究的目的:
- 为了确定参与BO病变的信号通路.
- 调查NET和BO发展之间的相关性.
主要方法:
- 对GEO数据集 (GSE52761,GSE137169) 的生物信息分析,包括差异基因表达,GO,KEGG和GSEA.
- 构建BO小鼠模型和分析肺组织.
- 用NETs处理的人类小气道上皮细胞 (SAEC) 的转录和生物信息学分析.
主要成果:
- 在BO中确定了115个上调的差异表达基因 (DEG),主要与炎症信号相关.
- 在BO小鼠模型中确认了NET形成和激活,中性粒细胞弹性酶 (NE) 和髓氧化酶 (MPO) 表达率升高.
- 在SAEC中与NETs相关的DEGs在炎症和上皮细胞转移到介质酶 (EMT) 途径中得到了丰富.
结论:
- NETs通过激活炎症和EMT相关途径,在BO病变发生中发挥重要作用.
- 这项研究为BO分子机制提供了新的见解.
- 研究结果表明,NETs是BO治疗的潜在治疗点.
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