从面包废物上的Bacillus amyloliquefaciens优化alpha-amylase,以通过响应表面方法有效处理工业废水和对织品进行测量
Basma T Abd-Elhalim1, Rawia F Gamal1, Salwa M El-Sayed2
1Department of Agricultural Microbiology, Faculty of Agriculture, Ain Shams University, Hadayek Shoubra, P.O. Box 68, Cairo, 11241, Egypt.
Scientific reports
|November 6, 2023
概括
这项研究使用Bacillus amyloliquificiens.使用面包废物可持续生产alpha-amylase酶. 优化的酶可以有效地处理废水和织品,展示工业潜力.
科学领域:
- 生物技术是生物技术.
- 酵素工程是什么? 酶工程是什么
- 废弃物价值化 废弃物价值化 废弃物价值化
背景情况:
- 食物浪费,特别是面包浪费,是一个重大的环境挑战.
- 像α-氨基酶这样的工业酶对于各种应用至关重要,但通常是通过昂贵的方法生产的.
- 从废物流中可持续生产酶提供了一个环保的替代方案.
研究的目的:
- 从废弃的面包废物中可持续地产生α-氨酶.
- 为了优化使用Bacillus amyloliquificiens的酶生产.
- 评估该酶在处理工业废水和织品加工中的有效性.
主要方法:
- 固态和浸泡发酵使用棕色和白色面包废物.
- 优化Bacillus amyloliquificiens发酵参数的优化.
- 在甲基酸盐珠上进行酶固定,以提高稳定性.
- 响应表面方法 (RSM) 和ANOVA的应用,以优化水解.
- 在以粉为基础的废水处理和织品加工中测试酶效率.
主要成果:
- 氨基酸菌表现出高粉水解和α-氨基酶活性.
- 在特定条件下 (37°C,24小时) 最佳的浸泡发酵产生了695.2个单位/毫升的α-氨酶.
- 固定化的酶在20次重复使用后保持了88.5%的活性.
- 使用RSM确定了最佳的水解条件 (pH 9,45°C).
- 该酶在15分钟内实现了96.3%的织品脱效率.
结论:
- 丢弃的面包废物是生产α-氨酸酶的可行和可持续基质.
- 优化的α-氨酶表现出了工业应用中理想的特性.
- 酶固定显著提高了它的可重复使用性和稳定性.
- 这种生物基酶为粉加工行业提供了一个有前途的绿色替代品.
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