在新生儿子中表征分枝后生
Meredith P Schuh1,2,3, Sunitha Yarlagadda4, Lyan Alkhudairy4
1Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, USA. Meredith.Schuh@cchmc.org.
Scientific reports
|November 6, 2023
概括
与小鼠不同的是,子表现出产后生与分支后生 (PBN) 弧形,为研究早产婴儿脏发育提供了一个新的模型.
科学领域:
- 发展生物学 发展生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
- 比较解剖学的比较解剖学
背景情况:
- 人类的神经发育在出生前就结束了,大多数神经元是在妊娠晚期形成的.
- 过早出生的婴儿需要产后的神经发育,极度早产与较低的脏数量有关.
- 识别患有产后发生的动物模型对于支持早产婴儿脏发育至关重要.
研究的目的:
- 为了调查子是否在出生后经历了分枝后生 (PBN).
- 描述子产生的时间和机制.
- 为了评估子作为研究产后PBN的模型.
主要方法:
- 从出生到产后49天,对子脏进行形态和免疫组织学评估.
- 用H&E和SIX1,SIX2,WT1,ZO-1和JAG1.1的抗体进行染色.
- 体利基的3D染和RNAScope用于转录映射 (Six1, Six2, Ret).
主要成果:
- 在SIX1之前,子SIX2表达在原体 (NPC) 中停止了,反映了人类的模式.
- 活跃的神经发生仅持续到产后3-5天 (PC35-36).
- 形态学分析显示,在出生后的子中,有证据表明PBN廊,但没有侧枝生.
结论:
- 子表现出产后的PBN道,尽管时间有限 (生命的前5天).
- 子是第一个表达SIX1的非灵长类动物.
- 子作为一个有价值的非灵长类模型,用于研究在产后 nefrogenic 利基.
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