基于RNA测序的功能性lncRNAs在心房的识别
Yangyang You1, Wei Wang1, Wenshu Zhu2
1Department of Cardiology, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230001, China.
BMC cardiovascular disorders
|November 6, 2023
概括
前庭动 (AF) 研究确定了与该疾病相关的特定长非编码RNA (lncRNAs). 该lncRNA AC009509.2显示了作为预测AF的新生物标志物的潜力.
科学领域:
- 心血管研究研究心血管研究
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 心房动 (AF) 是一种常见的心律失常,与中风和心力衰竭有关.
- 在AF背后的分子机制仍然不完全理解.
- 长非编码RNAs (lncRNAs) 已经成为心血管疾病的潜在参与者.
研究的目的:
- 与对照组相比,在AF患者中识别差异表达的lncRNA.
- 探索这些 lncRNAs 在 AF 发病过程中的潜在功能和相互作用.
- 评估已识别的 lncRNAs 对AF预测的诊断价值.
主要方法:
- 在AF患者和对照组的白细胞样本上进行了RNA测序 (RNA-seq).
- 不同表达分析确定了候选lncRNAs和mRNAs.
- 定量实时PCR (qRT-PCR) 验证了 lncRNA表达.
- 使用了基因本体学 (GO) 丰富,替代拼接 (AS) 分析和lncRNA-mRNA网络构建.
- 接收器操作特征 (ROC) 曲线分析评估的诊断值.
主要成果:
- 在AF患者和对照人群中,共确定了105种差异表达的lncRNAs.
- 在lncRNAs AC009509.2和LINC01781中,在AF中显示出显著的差异性表达.
- 多变量逻辑回归表明AC009509.2和左心房直径是AF的独立预测因素.
- 在ROC分析中,AC009509.2 (AUC=0.710) 和LINC01781 (AUC=0.654) 是AF的潜在诊断生物标志物.
结论:
- 这项研究突出了差异表达的lncRNAs在心房的作用.
- 鉴定出 lncRNA AC009509.2 是一个潜在的新生物标志物用于AF预测.
- 对lncRNA-mRNA相互作用的进一步调查可能会阐明AF的分子机制.
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