在非功能性垂体腺瘤中表达cAMP和与氧化酸化相关的lncRNAs
Mohammad Taheri1,2, Amir Nicknam3, Atena Bagan3
1Institute of Human Genetics, Jena University Hospital, Jena, Germany.
Journal of cellular and molecular medicine
|November 7, 2023
概括
非功能性垂体腺瘤 (NFPAs) 显示特定长非编码RNAs (lncRNAs) 的表达变化. 三种lncRNAs (DNAH17-AS1,LINC00706,MIR7-3HG) 在NFPA组织中显著下调,这表明它们在疾病发病过程中的潜在作用.
科学领域:
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 非功能性垂体腺瘤 (NFPA) 是常见的垂体瘤,具有显著的发病率.
- 了解NFPA开发背后的分子机制对于确定诊断和治疗点至关重要.
研究的目的:
- 与正常的垂体组织相比,在NFPA组织中识别差异表达的长非编码RNA (lncRNAs).
- 研究特定的lncRNAs (DNAH17-AS1,LINC00706,SLC25A5-AS1,CADM3-AS1,MIR7-3HG) 在NFPA病原发生中的潜在作用.
主要方法:
- 生物信息学分析以确定与cAMP和氧化酸化途径相关的候选 lncRNA.
- 定量逆转录聚合酶连锁反应 (RT-qPCR) 用于验证NFPA和相邻健康组织中的lncRNA表达水平.
- 接收器操作特征 (ROC) 曲线分析以评估诊断潜力.
主要成果:
- 与对照组相比,NFPA组织中的DNAH17-AS1,LINC00706和MIR7-3HG显著下调 (p <0.05).
- 对于这三个lncRNAs,ROC曲线分析显示了中等的诊断潜力 (AUC值~0.61-0.62).
- 在女性患者中,CADM3-AS1表达较低,在疾病持续时间小于一年的患者中,SLC25A5-AS1水平较低.
结论:
- 这项研究确定了NFPA组织中特定lncRNAs的失调.
- DNAH17-AS1,LINC00706和MIR7-3HG是潜在的生物标志物和调节器在非功能性垂体腺瘤的病变发生.
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