开发和全面验证一种新的液体染色学电化学检测方法,用于同时确定大鼠大脑中的九种甲醇胺
Saniye Özcan1,2, Aysun Geven3, Murat Kozanlı1
1Anadolu University Faculty of Pharmacy, Department of Analytical Chemistry, Eskişehir, Türkiye.
Turkish journal of pharmaceutical sciences
|November 7, 2023
概括
一种新的高性能液态色谱电化学检测 (HPLC-EC) 方法可以同时测量大鼠大脑中的九种神经递质. 这种经过验证的方法成功地确定了样本中最低的血清激素和最高的上腺素水平.
科学领域:
- 神经科学是一个神经科学.
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 化学神经传递对记忆和疼痛等大脑功能至关重要,其失调与神经系统疾病有关.
- 测量神经递质有助于理解疾病机制和治疗结果.
- 神经递质分析的挑战包括矩阵效应和色谱分离困难.
研究的目的:
- 开发和验证一种高性能液体染色学-电化学检测 (HPLC-EC) 方法,用于同时分析九种关键神经递质.
- 应用验证的方法来量化大鼠大脑样本中的神经递质.
主要方法:
- 根据FDA和EMA的指导方针建立了一个经过优化和完全验证的HPLC-EC方法.
- 使用Kinetex F5列实现了9种神经递质 (包括多巴胺,血清素和上腺素) 的分离.
- 分析使用HPLC与DECADE II EC检测器进行.
主要成果:
- 该方法表现出了卓越的选择性,所有分析品的相关系数>0.99.
- 发现检测和量化极限分别在0.010.03 ng/mL和3.049.13 ng/mL之间.
- 方法的稳定性和稳定性被统计评估.
结论:
- 开发和验证的HPLC-EC方法已成功应用于分析大鼠大脑样本中的神经递质.
- 分析显示,在研究的老鼠大脑中,血清激素 (SER) 的最低水平和诺亚上腺素的最高水平.
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