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Updated: Jul 11, 2025

Induction and Analysis of Epithelial to Mesenchymal Transition

Induction and Analysis of Epithelial to Mesenchymal Transition

Published on: August 27, 2013

在膀癌中,POLR3G通过PI3K/AKT信号通路促进EMT

Hualin Chen¹, Lin Ma¹, Wenjie Yang¹

¹Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

|November 7, 2023

概括

RNA聚合酶III子单元G (POLR3G) 在膀癌 (BLCA) 中升高,预测生存率差. 通过激活PI3K/AKT通路,POLR3G促进BLCA细胞迁移和入侵,建议它作为治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

RNA聚合酶III子单元G (POLR3G) 与各种癌症有关,但其在膀癌 (BLCA) 中的作用尚不清楚.
众所周知,POLR3G可以促进瘤发生,转移,癌症干和乳腺癌和肺癌中的化学抵抗.

研究的目的:

研究POLR3G在膀癌 (BLCA) 的生物功能和预后意义.
阐明POLR3G影响BLCA进展的潜在分子机制.

主要方法:

在BLCA组织中对POLR3G表达的生物信息分析以及与患者存活率的相关性.
在体外和体外功能测试,以评估POLR3G敲击对细胞迁移和侵入的影响.
西部斑点分析,以评估表皮层-介质细胞过渡 (EMT) 标记物的表达.
通过使用特定的抑制剂和激活剂,研究POLR3G对PI3K/AKT信号通路的激活.

主要成果:

在BLCA组织中,POLR3G的表达显著上调,并与患者的存活率下降有关.
在体外和体内,POLR3G缺乏减少了BLCA细胞的迁移和入侵.
对POLR3G的抑制降低了与EMT相关的介酶体标志物的调节.
POLR3G激活PI3K/AKT信号通路,该通路在BLCA中调解其亲迁移和亲侵入性影响.

结论:

POLR3G作为膀癌 (BLCA) 的独立预后预测器.
在BLCA中,POLR3G通过激活PI3K/AKT信号通路来促进上皮层-介质细胞转换 (EMT).
针对POLR3G或PI3K/AKT通路可能是BLCA的潜在治疗策略.

关键词:

在 AKT AKT 里面.在EMT中,EMT是EMT.在PI3K中,PI3K是指PI3K.在 POLR3G 里面.膀癌:膀癌是一种癌症.

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