对葡萄树干病原体中毒性因素的明显演变进行比较的泛基因分析
Jadran F Garcia1, Abraham Morales-Cruz1,2, Noé Cochetel1
1Department of Viticulture and Enology, University of California, Davis, Davis, CA, U.S.A.
Molecular plant-microbe interactions : MPMI
|November 7, 2023
概括
对葡萄树干病原体Eutypa lata,Neofusicoccum parvum和Phaeoacremonium minimum的基因组分析揭示了不同的毒性因子分布. 这些发现提高了对葡萄藤菌适应和疾病机制的理解.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 基因组学就是基因组学.
- 葡萄酒和葡萄酒的栽培.
背景情况:
- 葡萄树木的木质器官容易受到致病性菌菌的感染,从而造成重大损害.
- 像Eutypa lata,Neofusicoccum parvum和Phaeoacremonium minimum这样的树干病原体表现出不同的毒力.
- 了解病毒性基因的基因组基础对于管理葡萄藤疾病至关重要.
研究的目的:
- 研究三种主要葡萄树干病原体的基因组多样性和结构.
- 识别和分析它们的泛基因组内可疑毒性因子的分布.
- 阐明在L. vinifera中的真菌毒性背后的适应策略和机制.
主要方法:
- 50个EEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE 拉塔,N. 拉塔,N. N. 拉塔 parvum,以及P. 在P. 来自全球葡萄种植地区.
- 构建每个物种的核酸水平,无参考的泛基因组.
- 对基因组变异,基因家族动态和对毒性因素的选择压力的分析.
主要成果:
- 在核心,可用和私人基因组分数中观察到毒性因子的对比分布.
- 碳水化合物活性酶 (CAZymes) 在核心基因组中很常见,而生物合成基因集群在可用基因组中得到丰富.
- 可放弃的基因组显示了吉普赛转移元素的丰富和积极选择的毒性基因 (例如,多基基合成酶,糖转移酶).
结论:
- 这些葡萄藤病原体的基因组结构复杂,具有不同的适应策略.
- 毒性因子分布的变化有助于不同的病原性和疾病症状.
- 这项研究提供了对驱动葡萄藤真菌毒性的分子机制的关键见解.
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