在山羊生殖系干细胞中,UCHL1维持微环境恒温
Donghui Yang1, Mengfei Zhang1, Wenbo Chen1
1College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, China.
概括
乌比奎丁C终端酶L1 (UCHL1) 维持丸免疫恒温和精子生成. 破坏UCHL1会破坏SSC,增加炎症,并损害哺乳动物的生育能力.
科学领域:
- 生殖生物学 生殖生物学
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
背景情况:
- 精子干细胞 (SSC) 对男性生育和生殖系遗传至关重要.
- 由于压力或感染引起的丸免疫系统的破坏会导致不孕症,影响牲畜的健康.
- 乌比奎丁C终端化酶L1 (UCHL1) 在丸平衡中的作用尚不清楚.
研究的目的:
- 研究UCHL1对山羊SSC和小鼠丸的影响.
- 探索UCHL1的抗炎功能,在一个多种I:C诱导的炎症模型.
- 确定UCHL1在维持丸微环境平衡中的作用.
主要方法:
- 在山羊SSC与UCHL1敲击 (KD) 进行了体外研究.
- 在UCHL1 KD之后,对小鼠丸进行了体内研究.
- 采用聚I:C诱导的炎症模型来评估UCHL1的抗炎能力.
- 检查了TLR3/TBK1/IRF3信号通路和HSPA8的相互作用.
主要成果:
- 在山羊的SSC中,UCHL1 KD增加了亡和炎症,抑制了自我更新和增殖.
- 鼠标丸中的UCHL1 KD破坏了精卵管和精子细胞,升高了炎症标志物.
- 通过通过HSPA8对抗抑制TLR3/TBK1/IRF3通路,抑制了UCHL1的多I:C诱导的炎症.
- 在SSC中,UCHL1通过对抗HSPA8表现出抗病毒功能.
结论:
- 在SSC和精子生成中,UCHL1对于维持免疫平衡至关重要.
- 缺乏UCHL1会影响SSC的自我更新,增殖和精子生成,从而加剧炎症.
- UCHL1在SSC中具有抗病毒作用,为其在山羊精子生成中的机制提供了洞察力.
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