通过无处不在的Zelda因素对偶跳过条的多层次调节
Timothy R Bishop1, Pinar Onal2, Zhe Xu1
1Department of Biology, New York University, 100 Washington Square East, New York, NY 10003, USA.
概括
指蛋白Zelda (Zld) 对于早期发育至关重要,它调节基因表达模式. Zld直接和间接地控制着甚至跳过的基因.
科学领域:
- 发展生物学 发展生物学
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
背景情况:
- 泽尔达 (Zld) 是早期多索菲拉胚胎中囊胞转录的关键激活剂.
- 双规则基因甚至跳过 (eve) 呈现出一个七条纹的模式,这对发育至关重要.
- 基因调节包括用于激活的增强剂和用于抑制的缺口基因.
研究的目的:
- 调查Zld在调节偶跳过 (eve) 基因七条纹模式中的作用.
- 确定Zld对eve表达的影响是直接的还是间接的.
- 阐明Zld作为早期胚胎基因激活中的先驱蛋白的功能.
主要方法:
- 一个3.8kb的基因组片段的分析,其中包含了Eve基底促进剂和增强剂.
- 在同一监管区域内的正规Zld结合点的变异.
- 在体内实验评估基因表达在Zld操纵后的变化.
主要成果:
- 兹尔德促进了比科伊德激活剂和隙抑制剂与EVE调控片段的结合.
- 变异Zld网站减少了EVE表达,但没有完全废除条纹.
- 通过影响差距基因表达边界,Zld间接调节夏日条纹.
结论:
- 泽尔达作为先驱蛋白质,直接促进激活剂和抑制剂的结合.
- Zld对夏日条纹的调节是直接的和间接的,涉及间隙基因相互作用.
- 在Drosophila胚胎中,Zld建立了必要的空间模式,以适当地形成叶条纹.
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