相关实验视频

Updated: Jul 11, 2025

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016
11:04

Author Spotlight: Methods for Electroporation and Transformation Confirmation in Limosilactobacillus reuteri DSM20016

Published on: June 23, 2023

3.8K

通过内生可编程内核酶基基因组编辑工具开发Limosilactobacillus reuteri的底盘

Qiujin Guo1, Yiting Yan1, Zhenting Zhang1,2

  • 1National Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070 Hubei, P.R. China.

ACS synthetic biology
|November 7, 2023
PubMed
概括

研究人员开发了一种新的CRISPR-Cas9基因编辑系统,用于Limosilactobacillus reuteri,从而实现了高效的基因操纵. 这一突破加速了用于治疗疾病的活生物治疗产品 (LBPs) 的开发.

关键词:
生物膜是一种生物膜.克里斯普尔-Cas9是什么意思内源性内核核酶是内源性的.基因组编辑 基因组编辑在 LBPs 中,LBPs 是一个很好的选择.利莫西拉克托巴西勒斯 (Limosilactobacillus reuteri) 是一种植物.活生生的生物治疗产品益生菌的底盘是益生菌的底盘

更多相关视频

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity09:16

In Vitro Directed Evolution of a Restriction Endonuclease with More Stringent Specificity

Published on: March 25, 2020

7.3K
Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)08:21

Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

Published on: March 16, 2012

15.7K

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
6.0K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
17