蜘蛛的进化Homeobox基因谱通过并联和全基因组复制
Madeleine E Aase-Remedios1, Ralf Janssen2, Daniel J Leite1
1Department of Biosciences, Durham University, Durham, DH1 3LE, United Kingdom.
Molecular biology and evolution
|November 7, 2023
概括
蜘蛛中大多数重复的homeobox基因都源于全基因组重复 (WGD). 这个WGD事件塑造了蜘蛛基因进化,有证据表明在重复的NK基因中存在亚功能化.
科学领域:
- 进化生物学是进化的生物学.
- 基因组学就是基因组学.
- 分子生物学分子生物学
背景情况:
- 基因复制,包括全基因组复制 (WGD),是进化创新的关键驱动力.
- 之前的研究表明,在蜘蛛肺的祖先中存在WGD,这是由蜘蛛中重复的homeobox基因证明的.
- 从较小规模的重复中区分WGD衍生的重复物对于理解WGD对基因组进化的影响至关重要.
研究的目的:
- 调查蜘蛛中复制的homeobox基因的起源,区分WGD和其他复制事件.
- 在多个蜘蛛基因组中分析homeobox基因集群的分布和保护.
- 探索复制NK基因之间的功能分歧,特别是调控子功能化.
主要方法:
- 对八个染色体级蜘蛛基因组进行比较基因组学分析.
- 家庭盒基因集群分布和副本数量的表征.
- 检查基因内容和组织在重复的集群内.
- 与收获者 ортолог相比,对 *Parasteatoda tepidariorum* 中 NK 基因对应物的功能分析.
主要成果:
- 在蜘蛛中,大多数重复的homeobox基因与WGD起源一致.
- 在所有八种蜘蛛物种中都发现了两种保存的家庭盒基因集群 (Hox,NK,HRO,Irx,SINE) 的副本.
- 每个星团中的一个副本经常表现出退化,而另一个则保持更完整.
- 在蜘蛛中重复的NK基因之间发现了调控子功能化的证据.
结论:
- 这项研究证实,WGD对蜘蛛的homeobox基因库有显著的贡献.
- 这些发现突显了WGD后重复基因集群的差异性保留和退化.
- NK基因的调控子功能化证明了WGD在蜘蛛进化中的功能后果.
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