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Updated: Jul 11, 2025

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein
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Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

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在哺乳动物细胞中使用基因组集成的遗传密码扩展机器的双停止密码抑制

Birthe Meineke1, Johannes Heimgärtner1, Rozina Caridha1

  • 1Science for Life Laboratory, Karolinska Institutet, Department of Medical Biochemistry and Biophysics, Division of Genome Biology, 17165 Stockholm, Sweden; Ming Wai Lau Centre for Reparative Medicine, Stockholm Node, Karolinska Institutet, 17165 Stockholm, Sweden.

Cell reports methods
|November 7, 2023
PubMed
概括

基因编码的非正规氨基酸 (ncAAs) 可以通过 piggyBac 集成有效地纳入哺乳动物细胞. 这种方法使得活细胞中细胞表面受体的特定位点蛋白质标记和双光体修饰成为可能.

关键词:
CP: 生物技术 生物技术科普:分子生物学 分子生物学遗传密码扩展 遗传密码扩展珀色的抑制抑制生物对等标签的标签然后点击化学.哺乳动物细胞培养的方法非正规的氨基酸.随机基因组整合随机基因组整合停止编码子抑制.不自然的氨基酸非天然的氨基酸.

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