在部分观察到的基因调节过程中推断延迟
Hyukpyo Hong1,2, Mark Jayson Cortez3, Yu-Yu Cheng4
1Department of Mathematical Sciences, KAIST, Daejeon 34141, Korea.
我们开发了一种贝叶斯式MCMC方法,从部分观测中推断基因调节网络 (GRN) 动态. 这种方法准确地估计了未观察到的蛋白质的参数,克服了复杂生物系统中维度的诅咒.
科学领域:
- 系统生物学 系统生物学
- 计算生物学 计算生物学
- 分子生物学分子生物学
背景情况:
- 基因调控网络 (GRNs) 通过蛋白质相互作用控制细胞功能.
- 实验方法只能捕捉到GRN动态的一小部分.
- 从部分数据中推断GRN属性是具有挑战性的,因为复杂性和隐藏的过程.
研究的目的:
- 开发一种高效准确的方法,从有限的观测结果推断GRN参数.
- 解决隐藏组件的非马科夫模型中维度的诅咒.
- 估计未观察到的调节蛋白的动力和延迟参数.
主要方法:
- 开发了一个基于模拟的贝叶斯马尔科夫链蒙特卡洛 (MCMC) 方法.
- 采用了有效参数推断的近似概率.
- 使用了一种两步激活模型,其中包括未观察到的调节蛋白和观察到的光蛋白.
主要成果:
- 通过使用先前信息,成功推断出未观察到的调节蛋白的动态.
- 估计的转录,翻译和未观察到的蛋白质的目标搜索参数.
- 证明了该方法用于分析含有隐藏组件的非马科夫模型的可扩展性.
结论:
- 开发的贝叶斯式MCMC方法能够从部分观测中准确地推断GRN参数.
- 这种方法有效地处理与隐藏状态的非马科夫模型,推进系统生物学.
- 自由使用的R代码有助于在生物研究中得到更广泛的应用.
更多相关视频
11:36Author Spotlight: An Integrated Workflow to Study the Promoter-Centric Spatio-Temporal Genome Architecture in Scarce Cell Populations
Published on: April 21, 2023
12:54Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
Published on: March 7, 2018
相关概念视频
Structure of a Gene
However, only 1% of the DNA is composed of genes that encode proteins; the rest, 99% is non-coding DNA. This non-coding DNA performs...
Regulation of Expression at Multiple Steps
RNA Polymerase II Accessory Proteins
Regulation of Expression Occurs at Multiple Steps
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
What is Gene Expression?
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Chromatin Structure Regulates pre-mRNA Processing
The chromatin structure, especially...
