[通过固相提取与液态染色体质谱相结合在尿样中检测出三种烯代谢物]
1Key Laboratory of Health Risk Appraisal for Trace Toxic Chemicals of Zhejiang Province, Department of Physical and Chemical Testing, Ningbo Municipal Center for Disease Control and Prevention, Ningbo 315010, China.
概括
一种新的超高性能液态色谱-高分辨率质谱法 (UPLC-HRMS) 方法准确地测量尿液中的烯代谢物. 这种快速技术适用于监控工人的工人.
科学领域:
- 分析化学 分析化学
- 环境健康 环境健康
- 毒理学 毒理学 毒理学
背景情况:
- 烯暴露对职业暴露的工人造成健康风险.
- 监测尿液中的烯代谢物对于评估暴露水平至关重要.
- 现有的代谢物确定方法可能缺乏速度或灵敏度.
研究的目的:
- 开发和验证一种快速超高性能液体染色学高分辨率质谱法 (UPLC-HRMS) 方法.
- 量化三种关键的烯代谢物:二甲基马拉酸,三甲基马拉酸和四甲基马拉酸.
- 评估该方法是否适用于职业健康常规监测.
主要方法:
- 尿样使用固相提取 (SPE) 进行处理.
- 使用Accucore Ph/Hexyl柱实现了色谱分离.
- 分析使用电子喷雾电离和数据依赖的MS/MS获取进行.
主要成果:
- 该方法在1.0200.0μg/L范围内显示出出色的线性 (R2:0.99790.9993).
- 检测极限低至0.180.24微克/升.
- 峰值恢复率在83.0%~93.7%之间,相对标准偏差为2.2%~7.9%.
结论:
- 开发的UPLC-HRMS方法简单,快速,灵敏,准确.
- 这种方法可以同时确定尿液中主要的烯代谢产物.
- 该技术非常适合对职业暴露工人的生物监测.
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