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红鼓的改进的高质量参考基因组促进了与耐药性相关的基因探索过程

Yongshuang Xiao¹, Jing Liu², Jiehong Wei¹

¹Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Scientific data

|November 7, 2023

概括

产生了红鼓 (Sciaenops ocellatus) 的新型高质量基因组组,改善了连续性,并确定了抗压力的关键基因. 这为水产养殖的遗传改进和了解环境应激反应提供了基础.

科学领域:

水产养殖是水产养殖的一种方式.
基因组学就是基因组学.
海洋生物学海洋生物学

背景情况:

在全球范围内,Sciaenops ocellatus (红色鼓) 是一个重要的养殖鱼.
水产养殖实践面临极端天气和逃逸的挑战,影响海洋生物多样性.

研究的目的:

为了生成一个高质量的,染色体规模的基因组组装Sciaenops ocellatus.
为了确定与低体应激反应相关的基因,以改善水产养殖.

主要方法:

使用PacBio HiFi测序和Hi-C技术进行新的基因组组装.
基因组注释以识别蛋白质编码基因和重复序列.
权重基因共同表达网络分析 (WGCNA) 寻找枢纽基因.

主要成果:

实现了显著改进的基因组组装,具有28.30 Mb的N50连续性.
确定了257.36 Mb的重复序列和22845个编码蛋白质的基因.
发现了54个参与快速低体应激反应的枢纽基因.

结论:

高质量的红鼓基因组为分子育种和水产养殖中的遗传改进提供了宝贵的资源.
已识别的候选基因提供了对应激反应的分子机制的见解,这对于养殖鱼类的弹性至关重要.

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