相关实验视频
Updated: Jul 11, 2025

07:33
In vitro Organoid Culture of Primary Mouse Colon Tumors
Published on: May 17, 2013
35.1K
TCOF1通过稳定β-catenin促进结直肠癌的进展
Hyeseon Yun1,2, Ji-Eun You1,2, Jun Ki Hong1
1Asan Institute for Life Science, Asan Medical Center, Seoul, 05505, Republic of Korea.
Medical oncology (Northwood, London, England)
|November 7, 2023
概括
特雷克尔核糖体生物发生因子1 (TCOF1) 与β-catenin相互作用,影响结直肠癌 (CRC) 的进展. 调节TCOF1稳定性为CRC治疗提供了一个潜在的治疗策略.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 癌症研究 癌症研究
背景情况:
- 结肠直肠癌 (CRC) 是一个重大的全球健康挑战,死亡率高.
- Wnt/β-catenin通路在CRC病变发生过程中具有关键作用,β-catenin驱动下游基因表达.
- 尽管已知其在rDNA转录中的作用,但Treacle Ribosome Biogenesis Factor 1 (TCOF1) 在CRC中的作用仍然在很大程度上未被探索.
研究的目的:
- 研究TCOF1作为β-catenin在结直肠癌中的新型结合伙伴的潜在作用.
- 阐明TCOF1在CRC进展中的功能参与及其分子机制.
主要方法:
- 使用组织微阵列 (TMA) 对结直肠癌组织中TCOF1和β-catenin表达的分析.
- 通过西斑和qRT-PCR评估TCOF1对细胞增殖,殖民地形成和分子标记物的淘汰和过度表达效应.
- 使用ubiquitination测定和在体外/体内与坦基拉酶抑制剂NVP-TNKS656.6的相互作用研究对TCOF1-介导的β-catenin稳定性调节的研究.
主要成果:
- 在CRC样本中,TCOF1和β-catenin表达水平与瘤进展有显著的相关性.
- TCOF1调节 (敲击或过度表达) 显著影响了细胞增殖,改变了β-catenin酸化和cyclin D1水平.
- TCOF1通过蛋白质体降解来调节β-catenin的稳定性,其与NVP-TNKS656的相互作用使β-catenin不稳定.
结论:
- 已确定TCOF1和β-catenin是导致结直肠癌进展的重要危险因素.
- 针对TCOF1调节β-catenin稳定性,为结直肠癌治疗提供了一个有前途的治疗途径.
相关概念视频
Catenins
2.3K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.3K
Canonical Wnt Signaling Pathway
8.8K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.8K
TGF - β Signaling Pathway
7.4K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.4K
Tumor Progression
6.3K
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
6.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
mTOR Signaling and Cancer Progression
3.8K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.8K

