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不知功能的域581-9 负调节SNRK1激酶活性
Jennifer Bortlik1, Jost Lühle1, Saleh Alseekh2,3
1Plant Metabolism Group, Department of Plant Adaptation, Leibniz-Institute of Vegetable and Ornamental Crops (IGZ), Großbeeren 14979, Germany.
Plant physiology
|November 8, 2023
概括
一种植物蛋白,DUF581-9,在正常情况下抑制能量传感器SnRK1 (糖糖非发酵1相关蛋白激酶1). 它在饥饿期间的快速分解迅速激活了SnRK1以适应植物.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 生物化学 生物化学
背景情况:
- 糖分非发酵1 (SNF1) 相关的蛋白激酶1 (SnRK1) 是植物中关键的能量传感器,在能量缺乏期间调节细胞平衡的转录变化.
- 控制SnRK1激活的精确机制仍然不完全理解,尽管它有负面调节.
研究的目的:
- 研究阿拉比多普西斯的DOMAIN OF UNKNOWN FUNCTION (DUF581) 蛋白质,特别是DUF581-9/FCS类指3在调节SnRK1活动中的作用.
- 阐明DUF581-9影响SnRK1激活和植物对饥饿反应的分子机制.
主要方法:
- 研究了DUF581-9与Arabidopsis中SnRK1 (KIN10) 的催化子单元之间的相互作用.
- 在存在或缺少DUF581-9.9的情况下评估了SnRK1活性,T环酸化和KIN10自酸化.
- 研究了DUF581-9过度表达对植物对饥饿的适应及其蛋白质周转率的影响.
主要成果:
- DUF581-9直接与KIN10结合,通过防止双胞胎病毒反作用激酶 (GRIK) 依赖的T循环酸化来抑制SnRK1的激活.
- 在Arabidopsis中过度表达DUF581-9降低了SnRK1信号传递,降低了对饥饿的适应能力,降低了KIN10酸化.
- 在饥饿期间,DUF581-9蛋白经历了快速的蛋白质体降解,释放抑制并促进SnRK1的激活.
结论:
- 在能量充足的条件下,DUF581-9作为SnRK1活动的负调节剂.
- 在能源赤字期间,DUF581-9的快速循环提供了一个快速的SNRK1激活机制,独立于de novo蛋白质合成,有助于植物的适应.
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