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Updated: Jul 11, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Published on: September 8, 2012

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在CRISPR-Cas9 HNH核酶的基质独立激活路径

Jimin Wang1, Federica Maschietto2, Tianyin Qiu2

  • 1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, Connecticut.

Biophysical journal
|November 8, 2023
PubMed
概括

高保真Cas9 HNH核酶激活涉及三个构造. 构造2对于相互转换至关重要,突变通过阻断基质独立激活来增强裂变忠实性.

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