核因子-kappa B的受体激活剂富含 CD9 阳性细胞外囊泡,由骨质细胞释放
Shaobo Ruan1, Wellington J Rody2, Shivani S Patel3
1Department of Pharmaceutics, College of Pharmacy, Gainesville, FL 32610, USA.
Extracellular vesicles and circulating nucleic acids
|November 8, 2023
概括
核因子-kappa B (RANK) 的受体激活剂含有细胞外囊泡 (EVs) 是骨健康的关键. 这项研究发现RANK被选择性地丰富了来自骨质细胞的CD9阳性EV,提供治疗潜力.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 骨生物学 骨生物学 骨生物学
背景情况:
- 核因子-卡帕B (RANK) 的受体激活剂在骨代谢中起着至关重要的作用.
- 来自骨质细胞的细胞外囊泡 (EVs) 是潜在的治疗剂,用于骨代谢.
- 之前的研究在一小部分骨质细胞衍生的EV中检测到RANK.
研究的目的:
- 为了描述含有RANK的骨质细胞衍生的EVs的亚群.
- 调查RANK在特定EV子集中的选择性定位.
主要方法:
- 通过免疫光定位CD9和CD81在骨质细胞中.
- 传输电子显微镜用于EV可视化.
- 单颗粒干扰度反射成像传感器 (SP-IRIS) 和免疫亲和分离来检查EV中的RANK丰富.
主要成果:
- CD9主要位于骨质细胞等离子膜上,而CD81位于细胞质囊泡网络中.
- CD9和CD81阳性EVs很小 (56-83 nm) 和基本上是不同的群体.
- 在2%-4%的CD9阳性EV中检测到RANK,但在CD81阳性EV中没有,来自成熟的骨质细胞.
结论:
- 核因子-kappa B (RANK) 的受体激活剂被选择性地丰富在来自骨质细胞的CD9阳性细胞外囊泡 (EVs) 的子集中.
- 这是首次报告RANK在特定EV子集的选择性定位.
- 这些发现突显了CD9阳性EVs作为骨代谢的向治疗剂的潜力.
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