在Bacillus中的二次生物合成潜力的物种特异性
Qun-Jian Yin1, Ti-Ti Ying2, Zhen-Yi Zhou2
1Fourth Institute of Oceanography, Ministry of Natural Resources, Beihai, China.
Frontiers in microbiology
|November 8, 2023
概括
细菌物种表现出特定物种的生物合成基因集群 (BGCs),许多天然产品支架是单一物种独有的. 这突显了通过针对性方法在Bacillus.中发现新型化合物的潜力.
科学领域:
- 微生物学和自然产品发现
- 基因组学和生物信息学
背景情况:
- 细菌物种是众所周知的各种自然产品的生产者,但由于对生物合成基因集群 (BGC) 的知识有限,它们的全部生物合成潜力被低估了.
- 细菌物种之间BGC多样性和新奇性的变化阻碍了有效的自然产品发现.
研究的目的:
- 为了研究BGC在Bacillus属内分布的物种特异性.
- 分析来自Bacillus BGCs预测的二次代谢物的结构多样性和新性.
- 提供洞察力,以有针对性地发现来自Bacillus的新型天然产品.
主要方法:
- 在使用抗SMASH的6378个高质量的Bacillus基因组中预测了BGCs.
- 通过主要成分分析研究了BGC分布的物种特异性.
- 使用分子网络分析了二次代谢物的结构多样性和新性.
主要成果:
- 确定了特定物种的BGC分布,主要由 siderophore,III型多基合成酶 (T3PKS) 和 transAT-PKS BGCs驱动.
- 发现54.5%的基因集群 (GCFs) 和93.8%的代谢物支架仅限于单个Bacillus物种.
- 突出了Bacillus cereus,Bacillus thuringiensis和Bacillus velezensis,因为它们具有对特定物种NRPS和PKS天然产品的高潜力.
结论:
- 这项研究证实了Bacillus的二次生物合成潜力的显著物种特异性.
- 这些发现为针对性地发现Bacillus属内的新型自然产品提供了宝贵的见解.
- 这项研究强调了特定细菌物种中未开发的独特自然产品储备.
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