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m5U-GEPred:基于序列衍生和图形嵌入特征来预测RNA5-甲基尤里丁位点

Zhongxing Xu^1,2, Xuan Wang^3,4, Jia Meng^3,4,5

¹Department of Public Health, School of Medicine and Holistic Integrative Medicine, Nanjing University of Chinese Medicine, Nanjing, China.

Frontiers in microbiology

|November 8, 2023

概括

这项研究介绍了m5U-GEPred,这是一种用于识别RNA中的5-Methyluridine (m⁵U) 位点的新计算框架. 该方法结合了序列特征和图形嵌入,显著提高了RNA修饰的预测准确性.

科学领域:

分子生物学分子生物学
生物信息学是一种生物信息学.
计算生物学计算生物学

背景情况:

5-甲基尤里丁 (m⁵U) 是一种流行的转录后RNA修饰,对各种生物过程和疾病病原发生至关重要.
准确识别m⁵U位点对于理解RNA生物学和发现新的治疗点至关重要.

研究的目的:

开发和验证m5U-GEPred,用于识别m5U网站的预测框架.
通过将序列特征与基于图形嵌入的全球信息集成来增强m⁵U预测.

主要方法:

开发了m5U-GEPred,这是一个结合序列特征和图形嵌入的计算框架.
利用图形嵌入来捕获全球训练数据信息,补充本地序列特征.
评估了人类和酵母转录组和独立的牛津纳米孔技术 (ONT) 验证数据的性能.

主要成果:

在人类 (AUROC 0.984) 和酵母 (AUROC 0.985) 转录组上,m5U-GEPred与最先进的预测器相比表现优越.
在独立的ONT验证的m⁵U网站上实现了91.84%的预测准确性 (ACC).
图形嵌入的集成显著增强了预测能力.

结论:

m5U-GEPred为m5U网站识别提供了强大而准确的计算方法.
该框架的性能验证了结合序列和图形嵌入功能的实用性.
m5U-GEPred是RNA研究和发现基于RNA的治疗方法的宝贵工具.

关键词:

5 - 甲基尤里丁的使用.基因组RNA的修饰是RNA的修饰.图形嵌入图形嵌入.多个物种的多个物种.序列特征序列特征是一个特征.

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