使用微流体横向交流电泳 (TrACE) 测量单个粒子的电泳运动性和大小
M Hannah Choi1, Liu Hong2, Leonardo P Chamorro2
1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA. mhchoi1@seas.upenn.edu.
Lab on a chip
|November 8, 2023
概括
一种新的微流体横向交流电泳 (TrACE) 技术可以测量单个粒子的电荷和大小. 这种方法结合了粒子跟踪速度计和交流电泳,为生物和合成颗粒提供了更高的精度.
科学领域:
- 生物物理学的生物物理.
- 纳米技术纳米技术
- 分析化学 分析化学
背景情况:
- 精确测量单个粒子电荷和大小对于理解粒子相互作用和疾病发展至关重要.
- 目前用于测量单个粒子电泳运动 (μep) 的方法具有挑战性,同时测量尺寸和电荷的数量有限.
- 描述生物颗粒的物理性质可以将变化与疾病进展联系起来.
研究的目的:
- 引入和验证一种新技术,即微流体横向交流电泳 (TrACE),用于同时测量单粒子电泳运动 (μep) 和大小.
- 为了证明TRACE对合成颗粒和完整的哺乳动物细胞的多功能性.
- 建立TRACE作为粒子表征的潜在可检测工具.
主要方法:
- 微流体横向交流电泳 (TrACE) 结合了粒子跟踪速度计 (PTV) 与交流电泳.
- 粒子因横向应用的电波 (振幅为0.751.5V) 而在带有散流的微通道内振荡.
- 粒子轨迹由PTV记录,并使用准平衡模型来分析运动.
主要成果:
- TrACE精确测量了聚乙烯颗粒的电泳流动性 (μep) (0.532 μm),与ELS测量一致,通过平均值提高了精度.
- 使用布朗运动分析 (<2μm) 或图像分析 (≥2μm) 实现了同时测量粒子大小.
- 该技术成功分析了完整的哺乳动物B细胞,证明了它对生物样本的适用性.
结论:
- 微流体TRACE是一种新且有效的技术,用于同时测量单颗粒电泳流动性 (μep) 和大小.
- 该方法显示了高精度和准确性,与既定技术相比,可适应各种粒子类型,包括细胞.
- 作为一个便携式和多功能工具,TRACE在各种科学领域具有先进的粒子表征的前景.
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