相关实验视频

Updated: Jul 11, 2025

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Photoconversion of Purified Fluorescent Proteins and Dual-probe Optical Highlighting in Live Cells

Published on: June 26, 2010

化转换:准确和无毒的光转换的新兴参与者

Konstantinos Kalyviotis¹, Periklis Pantazis¹

¹Department of Bioengineering, Imperial College London, London, UK.

Journal of microscopy

|November 8, 2023

概括

化转换提供了一种用于将绿色转换为红色光蛋白的新方法,提高了精确的光学成像. 这种技术改善了单细胞标记和血统追踪,用于发育和疾病研究.

科学领域:

生物物理学的生物物理.
分子生物学分子生物学
光学成像技术的成像

背景情况:

可光转化光蛋白 (PFPs) 对于先进的成像技术至关重要.
传统的PFP转换方法使用紫色或近紫外线光,限制了应用.
据2015年报道的化转换使用蓝色和红色到远红色的光来进行PFP转换.

研究的目的:

审查化转换的原则和应用.
专注于其在单细胞标签和谱系追踪中的实用性.
讨论在原始化转换成像中的未来发展.

主要方法:

对光和PFPs的基本原理的审查.
谱系追踪技术的历史视角.
对原料转换的分子要求的分析.
双色高真实性血统追踪的演示. 双色高真实性血统追踪.

主要成果:

在PFP转换的传统方法上,化转换提供了优势.
该技术可以实现高保真,双色系谱追踪.
它为精确的光学成像提供了一个强大的工具.

结论:

化转换是光学成像和谱系追踪的重大进步.
该技术在研究发育和疾病进展方面具有广泛的潜在应用.
未来的发展将进一步增强原始化转换成像工具包.

关键词:

光蛋白质是一种光蛋白质.一个小鼠胚胎.照片转换照片转换在初始化转换转换过程中.斑马鱼是一种斑马鱼.

更多相关视频

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Cell Tracking Using Photoconvertible Proteins During Zebrafish Development

Published on: September 28, 2012

Single-cell Photoconversion in Living Intact Zebrafish

Single-cell Photoconversion in Living Intact Zebrafish

Published on: March 19, 2018

相关概念视频

Photochemical Electrocyclic Reactions: Stereochemistry

Photochemical Electrocyclic Reactions: Stereochemistry

The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation

Thermal and Photochemical Electrocyclic Reactions: Overview

Thermal and Photochemical Electrocyclic Reactions: Overview

Electrocyclic reactions are reversible reactions. They involve an intramolecular cyclization or ring-opening of a conjugated polyene. Shown below are two examples of electrocyclic reactions. In the first reaction, the formation of the cyclic product is favored. In contrast, in the second reaction, ring-opening is favored due to the high ring strain associated with cyclobutene formation.

Cycloaddition Reactions: MO Requirements for Photochemical Activation

Cycloaddition Reactions: MO Requirements for Photochemical Activation

Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.