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快速AST:有可能推断复杂的表型的耐药性机制

J Ligero-López, I Falces-Romero, A Aranda-Díaz

¹Emilio Cendejas-Bueno, Clinical Microbiology Department, Hospital Universitario La Paz, Madrid, Spain, Paseo de La Castellana 261, 28046 Madrid, Spain. ecendejas77@gmail.com.

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia

|November 8, 2023

概括

加快Pheno®系统 (AAC) 显示出对抗微生物敏感性测试 (AST) 的承诺,但与产生卡巴酶的グラム阴性细菌作斗争. 需要进一步的研究才能准确地推断出电阻机制.

科学领域:

临床微生物学临床微生物学
抗微生物耐药性抗微生物耐药性
诊断技术诊断技术的使用

背景情况:

自动化抗微生物敏感性测试 (AST) 系统加速血液感染的结果.
由于治疗选择有限,产生卡巴酶的グラム阴性细菌构成重大威胁.

研究的目的:

评估Accelerate Pheno®系统 (AAC) 对抗微生物敏感性测试 (AST) 的准确性.
为了比较AAC性能与MicroScanWalkAway系统 (MWS) 和卡巴酶生成分离物的磁盘扩散.
评估AAC在具有挑战性的グラム阴性细菌中推断抗药机制的能力.

主要方法:

使用19种产生卡巴酶的グラム阴性细菌分离物的比较研究.
对AAC,MWS和磁盘扩散进行的表型抗微生物敏感性测试 (AST).
对梅罗和埃尔塔的基本协议 (EA) 和类别协议 (CA) 的分析.

主要成果:

与MWS相比,AAC对美罗 (EA 84.2%) 和埃尔塔 (EA 88.2%,CA 64.7%) 显示出适度的一致性.
磁盘扩散显示了两个代理商的较低类别协议 (CA).
在推断碳酶生成分离物的耐药性机制时,AAC的准确性显著下降.

结论:

该AAC系统需要进一步验证可靠的AST在生产卡巴酶的グラム阴性细菌中.
目前的局限性阻碍了准确的MIC确定和阻力机制推断.
未来的研究应该包括新的抗生素和更大的隔离队列,以进行全面的评估.

关键词:

加快Pheno®系统的加速.格拉姆阴性细菌是什么?碳烯的质量非常大.

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