肝脏糖原和糖脂的运动训练适应需要肝脏AMP激活蛋白激酶在小鼠
Curtis C Hughey1,2, Deanna P Bracy2,3, Ferrol I Rome1
1Division of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, Minnesota, United States.
American journal of physiology. Endocrinology and metabolism
|November 8, 2023
概括
AMP激活蛋白激酶 (AMPK) 对于运动训练至关重要,以改善肝脏葡萄糖和脂质代谢. 没有AMPK,肝脏无法增加糖原或适当调整脂质,影响能量调节和脂肪肝疾病风险.
科学领域:
- 运动生理学 运动生理学
- 代谢调节 代谢调节 代谢调节
- 肝脏新陈代谢 肝脏新陈代谢
背景情况:
- 定期炼可以改善葡萄糖和脂质代谢,减轻脂肪肝等疾病.
- 剧烈的运动会激活肝脏中的AMP激活蛋白激酶 (AMPK),促进脂质氧化和保存ATP.
- AMPK在调解肝脏适应运动训练,特别是葡萄糖和脂质代谢中的作用尚未完全阐明.
研究的目的:
- 测试AMPK对于肝脏适应运动训练所必需的假设.
- 为了研究肝脏特异性AMPK淘汰对运动期间葡萄糖和脂质代谢的影响.
- 确定AMPK在训练诱导的肝糖原,葡萄糖生产和脂质谱的变化中的作用.
主要方法:
- 肝脏特异性AMPKα1α2淘汰赛小鼠和产后对照小鼠接受了久坐不动和运动训练方案.
- 肝脏营养流,代谢物和分子调节剂在休息状态和急性运动期间被评估.
- 肝脏糖原的量化,糖原溶解,葡萄糖生成,和脂类物种 (二甲基甘油,三甲基甘油).
主要成果:
- 运动训练在对照小鼠中增加了肝糖原,但在AMPK淘汰赛小鼠中没有.
- 在运动后训练期间,AMPK淘汰赛小鼠表现出减少的葡萄糖分解,葡萄糖生产和循环葡萄糖.
- AMPK 缺失减弱了肝脏二甲基甘油的训练诱导下降和脂肪酸组成的改变,同时增加了三甲基甘油.
结论:
- 对于运动训练引起的肝糖原增加,AMPK是必需的,肝糖原对于运动期间的葡萄糖供应至关重要.
- AMPK调解训练诱导的肝糖脂减少,独立于TCA循环流量,并影响脂肪酸代谢.
- 这些发现突显了AMPK在肝脏适应运动方面的关键作用,这对脂肪肝疾病的管理有影响.
相关概念视频
cAMP-dependent Protein Kinase Pathways
6.4K
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...
6.4K
Exercise and Muscle Performance
1.5K
Exercise induces a range of adaptations in muscle tissue, depending on the type and duration of activity. Such physical training can be broadly categorized into two types: endurance exercises and resistance exercises.
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
Endurance exercises
Endurance exercises involve running, swimming, or cycling, which require repetitive movements with low force output. When a person engages in endurance exercise, a few noticeable changes occur in their skeletal muscles. For instance, the number of capillaries...
1.5K
Muscle Recovery and Fatigue
2.1K
Muscle fatigue refers to the decline in a muscle's ability to maintain the force of contraction after prolonged activity. It primarily stems from changes within muscle fibers. Even before experiencing muscle fatigue, one may feel tired and have the urge to stop the activity. This response, known as central fatigue, occurs due to changes in the central nervous system, namely the brain and spinal cord. While there is no single mechanism that induces fatigue, it may serve as a protective...
2.1K
Metabolic States of the Body: Fasting and Starvation
1.4K
During the initial hours of fasting, the body uses up its glycogen stores as an energy source. Once these glycogen reserves are depleted, the body begins breaking down stored triglycerides and structural proteins. During this stage, glycerol becomes a key substrate for gluconeogenesis, while free fatty acids undergo beta-oxidation to provide energy for tissues, such as skeletal muscle. In the fasting state, the body spares protein breakdown as much as possible to conserve muscle and structural...
1.4K
Metabolic States of the Body: The Postabsorptive State
331
The postabsorptive state usually starts about four hours after a meal and lasts until the next meal is eaten. During this time, the digestive system stops absorbing nutrients, and the body uses stored energy reserves to maintain stable blood glucose levels.
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
Initially, glycogen stored in the liver is broken down to release glucose into the bloodstream, while glycogen in the muscles is broken down to supply glucose for energy directly within the muscle cells. As glycogen stores diminish,...
331


